Objective. To investigate the effect of isoflurane (ISO) on the proliferation, apoptosis, and inflammatory response of lipopolysaccharide- (LPS-) induced normal human astrocytes (NHAs) by regulating the miR-206/BDNF axis. Methods. NHA proliferation activity was measured by MTT; NHA apoptotic rates were measured by Annexin V-FITC/PI; western blotting was used to measure the BDNF expression; ELISA was used to measure the IL-6, IL-1β, and TNF-α expression in NHAs; qPCR was used to measure the expressions of miRNAs that are related to NHAs proliferation and apoptosis; dual-luciferase reporter was constructed to validate the targeting relationship between miR-206 and BDNF. Results. LPS increased the proliferation activity and decreased the apoptosis rate of NHAs which were effectively reversed by the ISO (); LPS significantly inhibited the expression of miRNAs related to proliferation and apoptosis in NHAs (, ), whereas ISO significantly increased the expression of miR-206 () by downregulating the expression of BDNF, thus inhibiting NHA proliferation and inflammatory response and enhancing apoptosis. Conclusion. ISO can inhibit the expression of BDNF by upregulating the expression of miR-206, thereby inhibiting the proliferation and inflammatory response of NHAs and promoting its apoptosis.

中文翻译：

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异氟烷通过miR-206 / BDNF轴调节脂多糖诱导的人星形胶质细胞的增殖，凋亡和炎症反应。

目标。为了研究异氟烷（ISO）通过调节miR-206 / BDNF轴对脂多糖（LPS-）诱导的正常人星形胶质细胞（NHA）增殖，凋亡和炎症反应的影响。方法。用MTT法测定NHA的增殖活性。用膜联蛋白V-FITC / PI测量NHA的凋亡率。用western blotting检测BDNF的表达。ELISA法检测NHAs中IL-6，IL- 1β和TNF- α的表达。qPCR用于测量与NHA增殖和凋亡相关的miRNA的表达。构建双荧光素酶报告基因以验证miR-206和BDNF之间的靶向关系。结果。LPS可增加NHA的增殖活性并降低其凋亡率，而ISO可以有效逆转LHA（）; LPS显着抑制了与NHA中增殖和凋亡相关的miRNA的表达（， ），而ISO显着提高了miR-206的表达（），通过下调BDNF的表达，从而抑制NHA增殖和炎症反应并增强细胞凋亡。结论。ISO可以通过上调miR-206的表达来抑制BDNF的表达，从而抑制NHA的增殖和炎症反应，并促进其凋亡。