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Metabolite Profiling of Sorghum Seeds of Different Colors from Different Sweet Sorghum Cultivars Using a Widely Targeted Metabolomics Approach
International Journal of Genomics ( IF 2.6 ) Pub Date : 2020-03-04 , DOI: 10.1155/2020/6247429
Yaxing Zhou 1 , Zhenguo Wang 2 , Yan Li 2 , Zhigang Li 1 , Hui Liu 3 , Wei Zhou 1
Affiliation  

Sweet sorghum (Sorghum bicolor) is one of the most important cereal crops in the world with colorful seeds. To study the diversity and cultivar-specificity of phytochemicals in sweet sorghum seeds, widely targeted metabolomics was used to analyze the metabolic profiles of the white, red, and purple seeds from three sweet sorghum cultivars Z6, Z27, and HC4. We identified 651 metabolites that were divided into 24 categories, including fatty acids, glycerolipids, flavonoids, benzoic acid derivatives, anthocyanins, and nucleotides and its derivatives. Among them, 217 metabolites were selected as significantly differential metabolites which could be related to the seed color by clustering analysis, principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA). A significant difference was shown between the red seed and purple seed samples, Z27 and HC4, in which 106 were downregulated and 111 were upregulated. The result indicated that 240 metabolites were significantly different, which could be related to the purple color with 58 metabolites downregulated and 182 metabolites upregulated. And 199 metabolites might be involved in the red phenotype with 54 downregulated and 135 upregulated. There were 45 metabolites that were common to all three cultivars, while cyanidin O-malonyl-malonyl hexoside, cyanidin O-acetylhexoside, and cyanidin 3-O-glucosyl-malonylglucoside were significantly upregulated red seeds, which could be the basis for the variety of seed colors. Generally, our findings provide a comprehensive comparison of the metabolites between the three phenotypes of S. bicolor and an interpretation of phenotypic differences from the point of metabolomics.

中文翻译:

广泛目标代谢组学方法从不同甜高粱品种中提取不同颜色的高粱种子的代谢物

甜高粱(双色高粱))是世界上最重要的谷物作物,种子色彩艳丽。为了研究甜高粱种子中植物化学物质的多样性和品种特异性,使用了广泛靶向的代谢组学方法分析了三个甜高粱品种Z6,Z27和HC4的白色,红色和紫色种子的代谢谱。我们鉴定了651种代谢物,将其分为24类,包括脂肪酸,甘油脂,类黄酮,苯甲酸衍生物,花色苷以及核苷酸及其衍生物。其中,通过聚类分析,主成分分析(PCA)以及正交信号校正和偏最小二乘判别分析(OPLS-DA),选择了217种代谢物作为显着差异的代谢物,这些代谢物可能与种子的颜色有关。在红色种子样品和紫色种子样品Z27和HC4之间显示出显着差异,其中下调了106个,上调了111个。结果表明240种代谢产物之间存在显着差异,这可能与紫色有关,其中58种代谢产物被下调而182种代谢产物被上调。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。通常,我们的发现提供了三种表型之间的代谢物的全面比较。其中下调了106个,上调了111个。结果表明240种代谢产物之间存在显着差异,这可能与紫色有关,其中58种代谢产物被下调而182种代谢产物被上调。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。通常,我们的发现提供了三种表型之间的代谢物的全面比较。其中下调了106个,上调了111个。结果表明240种代谢产物之间存在显着差异,这可能与紫色有关,其中58种代谢产物被下调而182种代谢产物被上调。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。通常,我们的发现提供了三种表型之间的代谢物的全面比较。结果表明240种代谢产物之间存在显着差异,这可能与紫色有关,其中58种代谢产物被下调而182种代谢产物被上调。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。通常,我们的发现提供了三种表型之间的代谢物的全面比较。结果表明240种代谢产物之间存在显着差异,这可能与紫色有关,其中58种代谢产物被下调而182种代谢产物被上调。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。一般而言,我们的发现提供了三种表型之间的代谢物的综合比较。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。通常,我们的发现提供了三种表型之间的代谢物的全面比较。红色表型可能涉及199种代谢物,其中54种下调而135种上调。这三个品种共有45种代谢产物,而花青素O-丙二酰基-丙二酰基己糖苷,花青素O-乙酰基己糖苷和花青素3-O-葡糖基-丙二酰基葡糖苷是显着上调的红色种子,这可能是其变种的基础。种子的颜色。通常,我们的发现提供了三种表型之间的代谢物的全面比较。双色链球菌和从代谢组学角度解释表型差异。
更新日期:2020-03-04
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