A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of Blumea balsamifera, including 17 flavonoids and 1 phenylethanone. The B. balsamifera extraction was separated by a Kromasil C₁₈ column (250 × 4.6 mm, 5 μm) with a binary gradient mobile phase consisting of acetonitrile and 0.2% aqueous acetic acid. A photodiode array detector (PDA) was used to record the signals of investigated constituents. The linearity, sensitivity, stability, precision, and accuracy of the established assay methods were assessed to meet the requirements of quantitative determination. Samples extracted by reflux in 25 mL of 80% methanol for 30 minutes were selected for the extraction method. The 18 compounds were accurately identified by comparing with the reference compounds. The purity of each peak was confirmed by the base peak in the mass spectrum. The contents of 18 compounds in Blumea samples from four different regions were successfully determined. The results also showed that 3,3′,5,7-tetrahydroxy-4′-methoxyflavanone was the most abundant constituent, which could be used as a potential chemical marker for quality control of B. balsamifera and Chinese patent medications containing B. balsamifera herb.

中文翻译：

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高效液相色谱-光电二极管阵列检测仪同时定量测定凤仙花（Ai-Na-Xiang，Sembung）中的多酚类化合物

开发了一种高效液相色谱方法，用于同时定量香脂梅叶中的18种多酚化合物，包括17种类黄酮和1种苯乙酮。所述B.香脂提取物通过在Kromasil下分离₁₈柱（250×4.6毫米，5  μm）具有由乙腈和0.2％乙酸水溶液组成的二元梯度流动相。光电二极管阵列检测器（PDA）用于记录所研究成分的信号。对已建立的测定方法的线性，灵敏度，稳定性，精密度和准确性进行了评估，以满足定量测定的要求。选择在25 mL 80％甲醇中回流30分钟提取的样品作为提取方法。通过与参考化合物进行比较，可以准确鉴定出18种化合物。每个峰的纯度由质谱中的基本峰确定。成功测定了来自四个不同区域的布鲁梅亚样品中18种化合物的含量。结果还显示3,3'，5,7-四羟基-4'-甲氧基黄酮是最丰富的成分，B. balsamifera和含有B. balsamifera草药的中成药。