Radix Polygoni Multiflori (RPM) has been widely used to treat various diseases in Asian countries for many centuries. Although, stilbenes and anthraquinones, two major components of RPM, show various bioactive effects, it has been speculated that the idiosyncratic hepatotoxicity induced by RPM may be related to these constituents. However, information on the pharmacokinetics of stilbenes and anthraquinones at a subtoxic dose of RPM is limited. A simple and sensitive UPLC-MS/MS bioanalytical method for the simultaneous determination of 13 ingredients of RPM, including chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, torachrysone-8-O-glucoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside, in rat plasma was established. Acetonitrile was employed to precipitate the plasma with appropriate sensitivity and acceptable matrix effects. Chromatographic separation was performed using a waters HSS C18 column with a gradient elution using water and acetonitrile both containing 0.025% formic acid within a run time of 9 min. The constituents were detected in negative ionization mode using multiple reaction monitoring. The method was fully validated in terms of selectivity, linearity, accuracy, precision, recovery, matrix effects, and stability. The lower limit of quantitation of the analytes was 0.1–1 ng/mL. The intrabatch and interbatch accuracies were 87.1–109%, and the precision was within the acceptable limits. The method was applied to a pharmacokinetic study after oral administration of RPM extract to rats at a subtoxic dose of 36 g/kg.

中文翻译：

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大鼠血浆中何首乌中13种成分的同时测定及其在药代动力学研究中的应用

何首乌（RPM）已被广泛用于治疗亚洲国家的多种疾病，已有多个世纪的历史了。尽管斯蒂芬斯和蒽醌是RPM的两个主要成分，它们显示出各种生物活性，但据推测RPM诱导的特异肝毒性可能与这些成分有关。然而，关于亚砜剂量下的对苯二酚和蒽醌的药代动力学的信息是有限的。一种简单而灵敏的UPLC-MS / MS生物分析方法，可同时测定13种RPM成分，包括菜油酚，大黄素，芦荟大黄素，大黄酸，physcion，questin，citreoroseosein，questinol，2,3,5,4'-四羟基sti -2-O- β -D-葡糖苷，Torrrysone-8-O-葡糖苷，Chrysophanol-8-O- β -D-葡糖苷，大黄素8-O- β-D-葡萄糖苷和physcion-8-O- β在大鼠血浆中建立了-D-葡萄糖苷。使用乙腈以适当的灵敏度和可接受的基质效应沉淀血浆。使用waters HSS C18色谱柱进行色谱分离，并在9分钟的运行时间内使用均包含0.025％甲酸的水和乙腈进行梯度洗脱。使用多反应监测以负电离模式检测成分。该方法在选择性，线性，准确度，精密度，回收率，基质效应和稳定性方面得到了充分验证。分析物的定量下限为0.1–1 ng / mL。批内和批间精度为87.1–109％，且精度在可接受的范围内。