Objective. Propofol is a classical anesthetic and induces consciousness loss, and gamma-aminobutyric-acid-type-A (GABA-A) receptor is its target. Righting reflex is associated with conscious response. The nucleus basalis (NB) acts as a major relay between the reticular activating system and the frontal cortex (FC). Propofol may mediate righting reflex by affecting GABA-A receptor in NB. Methods. Fifty male SD rats (250-350 g) were divided into parts I and II. In part I, 20 male SD rats were randomly divided into control group (CG) and NB-lesion group (NG, ibotenic acid-induced NB lesion). In part II, 30 male SD rats were treated with saline (0.9% NaCl, SG group), muscimol (a GABA-A receptor agonist, MG group), and gabazine (a GABA-A receptor antagonist, GG group) in NB, respectively. Two weeks later, the activity of the rats was measured between CG and NG groups. The rats were intravenously injected with propofol (50 mg/kg/h) to test the time of loss of righting reflex (LORR) in all rats. When LORR occurred, the rats received single administration of propofol (12 mg/kg) to measure the time of return of righting reflex (RORR). Electroencephalogram (EEG) activity of the frontal cortex (FC) was recorded. Results. The numbers of NB neurons were reduced by 44% in the NG group compared to the CG group () whereas the activity of rats was reduced a little in the NG group when compared with the CG group, but the statistical difference was insignificant (). The dose-response curve of propofol shifted to the left in the NG group, and the statistical difference for the time of LORR was insignificant between the two groups (). However, the time of RORR and FC delta power increased in the NG group compared with the CG group (). In part II, the time of RORR and FC powder increased in the MG group when compared to the SG group while reverse results were observed in the GG group (). There was no significant influence on the time of LORR and ED50 among the three groups (). Conclusions. The unilateral NB lesion increased the recovery time and FC delta power, and the NB region might be involved in the emergence after propofol administration. Propofol plays a crucial role for causing conscious loss by affecting GABA-A receptor in NB.

中文翻译：

---

异丙酚通过影响大鼠基底核 GABA-A 受体导致意识丧失

客观的。丙泊酚是一种经典的麻醉剂，会导致意识丧失，而 γ-氨基丁酸 A 型 (GABA-A) 受体是其靶标。翻正反射与有意识的反应有关。基底核 (NB) 充当网状激活系统和额叶皮层 (FC) 之间的主要中继。异丙酚可能通过影响 NB 中的 GABA-A 受体介导翻正反射。方法. 将 50 只雄性 SD 大鼠 (250-350 g) 分为第一部分和第二部分。在第一部分，将20只雄性SD大鼠随机分为对照组（CG）和NB-病变组（NG，ibotenic acid-induced NB lesion）。在第二部分中，30 只雄性 SD 大鼠在 NB 中分别用生理盐水（0.9% NaCl，SG 组）、muscimol（一种 GABA-A 受体激动剂，MG 组）和 gabazine（一种 GABA-A 受体拮抗剂，GG 组）处理，分别。两周后，在 CG 组和 NG 组之间测量大鼠的活动。大鼠静脉注射丙泊酚（50 mg/kg/h）以测试所有大鼠的翻正反射消失时间（LORR）。发生LORR时，大鼠单次给予丙泊酚（12mg/kg）以测量翻正反射恢复时间（RORR）。记录额叶皮层 (FC) 的脑电图 (EEG) 活动。结果. 与 CG 组相比，NG 组的 NB 神经元数量减少了 44%（( _）。NG组丙泊酚剂量反应曲线左移，两组LORR时间差异无统计学意义。）。然而，与 CG 组相比，NG 组的 RORR 和 FC delta power 时间增加（）。在第二部分中，与SG组相比，MG组的RORR和FC粉末时间增加，而GG组观察到相反的结果（）。三组之间对LORR时间和ED50时间无显着影响（）。 结论。单侧NB病变增加了恢复时间和FC delta功率，并且NB区域可能参与丙泊酚给药后的出现。异丙酚通过影响 NB 中的 GABA-A 受体，在引起意识丧失方面起着至关重要的作用。