Aging Cartilage in Wild-Type Mice: An Observational Study.,CARTILAGE

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Aging Cartilage in Wild-Type Mice: An Observational Study.
CARTILAGE ( IF 2.7 ) Pub Date : 2020-06-01 , DOI: 10.1177/1947603520926713
Joulnar Akoum _{1,

2} , Khadija Tahiri _{1,

2} , Marie-Thérèse Corvol _{1,

2} , Didier Borderie _{1,

3,

4} , François Étienne _{1,

2} , François Rannou _{1,

5,

6} , Christelle Nguyen _{1,

5,

6}

Affiliation

Objective

To describe the spontaneous evolution of age-related changes affecting knee joint articular cartilage, walking speed and a serum biomarker of cartilage remodeling in C57BL/6-JRj wild-type male mice.

Design

Histological changes were assessed by the Osteoarthritis Research Society International (OARSI) score (0=normal, 6=vertical clefts/erosion to the calcified cartilage extending >75% of the articular surface) in newborn, 1-week- and 1-, 3-, 6-, 9- and 12-month-old C57BL/6-JRj wild-type male mice, walking speed by the Locotronic system, and serum C-terminal telopeptide of type II collagen (CTX-II) content by ELISA in 1-, 3-, 6-, and 9-month-old C57BL/6-JRj wild-type male mice.

Results

Mean (SD) OARSI score significantly increased from 0.2 (0.3) to 1.3 (0.6) (p=0.03) between 1 and 3 months of age and from 1.3 (0.6) to 3.3 (0.6) (p=0.04) between 3 and 6 months of age. Mean walking speed was stable between 1 and 6 months of age but significantly decreased from 11.4 (1.8) to 3.2 (0.8) cm.s-1 (p=0.03) between 6 and 9 months of age. Serum CTX-II content was maximal at 1 month of age, then decreased from 12.2 (8.5) to 2.4 (8.4) pg/ml (p=0.02) between 1 and 3 months of age, remaining low and stable thereafter.

Conclusions

C57BL/6-JRj wild-type male mice showed continuously increasing osteoarthritic changes but delayed decreasing walking speed with age. These variations were maximal between 3 and 9 months of age. Maximal serum CTX-II content preceded these changes.

中文翻译：

野生型小鼠的老化软骨：一项观察性研究。

客观的

描述影响 C57BL/6-JRj 野生型雄性小鼠膝关节软骨、步行速度和软骨重塑血清生物标志物的年龄相关变化的自发演变。

设计

组织学变化通过新生儿、1 周和 1、3 -、6、9 和 12 个月大的 C57BL/6-JRj 野生型雄性小鼠，通过 Locotronic 系统检测步行速度，通过 ELISA 检测血清 II 型胶原蛋白 C 末端端肽 (CTX-II) 含量1、3、6 和 9 个月大的 C57BL/6-JRj 野生型雄性小鼠。

结果

平均 (SD) OARSI 评分在 1 至 3 个月之间从 0.2 (0.3) 显着增加至 1.3 (0.6) ( p =0.03)，在 3 至 6 个月之间从 1.3 (0.6) 显着增加至 3.3 (0.6) ( p =0.04)月龄。平均步行速度在 1 到 6 个月之间保持稳定，但在 6 到 9 个月之间从 11.4 (1.8) 显着下降到 3.2 (0.8) cm.s-1 ( p =0.03)。血清 CTX-II 含量在 1 个月大时最高，然后在 1 至 3 个月大时从 12.2 (8.5) 降至 2.4 (8.4) pg/ml ( p =0.02)，此后保持低水平且稳定。