Competing Interactions of Fatty Acids and Monoglycerides Trigger Synergistic Phospholipid Membrane Remodeling.,The Journal of Physical Chemistry Letters

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Competing Interactions of Fatty Acids and Monoglycerides Trigger Synergistic Phospholipid Membrane Remodeling.
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2020-06-01 , DOI: 10.1021/acs.jpclett.0c01138
Bo Kyeong Yoon _{1,

2} , Soohyun Park ₁ , Gamaliel J Ma ₁ , Kavoos Kolahdouzan _{1,

3} , Vladimir P Zhdanov ₄ , Joshua A Jackman ₂ , Nam-Joon Cho ₁

Affiliation

Using quartz crystal microbalance-dissipation and time-lapse fluorescence microscopy, we demonstrate that adding mixtures of lauric acid (LA) and glycerol monolaurate (GML), two of the most biologically active antimicrobial fatty acids and monoglycerides, to a supported lipid bilayer triggers concurrent tubule and bud formation, which unexpectedly results in synergistic phospholipid membrane remodeling that far exceeds the effects of GML or LA alone. Together, GML and LA drive pearling instability, dynamic transformation of buds into tubules and vice versa, and extensive membrane lysis. The most pronounced effects occurred with equimolar concentrations of GML and LA, highlighting that synergistic membrane disruption arises from competition for the lipid supply to buds and tubules and an inability to relieve membrane strains. These findings offer a conceptually new model to explain how fatty acid and monoglyceride interactions can trigger phospholipid membrane remodeling events relevant to various biophysical and biological systems.

中文翻译：

脂肪酸和甘油单酸酯的竞争性相互作用触发了磷脂膜的协同重塑。

使用石英微平衡耗散和延时荧光显微镜观察，我们证明了将月桂酸（LA）和甘油单月桂酸酯（GML）（两种生物活性最高的抗微生物脂肪酸和甘油单酸酯）的混合物添加到支持的脂质双层中可同时触发小管和芽的形成，意外地导致了磷脂膜的协同重塑，远远超过了仅GML或LA的作用。GML和LA共同驱动珠光不稳定，芽动态转化为小管（反之亦然）以及广泛的膜裂解。最明显的影响发生在等摩尔浓度的GML和LA时，突显出膜协同作用的破坏是由于竞争向芽和小管供应脂质而无法缓解膜应变。

更新日期：2020-07-02

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