Motivation: The downstream biological analysis of DIA-based proteomic data including protein abundance statistics, differential expression, functional annotation and enrichment analysis for variety databases is a crucial part for proteomic research, but few integrated tools and solutions are available, which leads to complex analytical processes and irreproducible analytical results. Results: We developed obaDIA, an integrated one-step pipeline for biological analysis from DIA data. ObaDIA is a fast tool which generate highly visualized, comprehensive and repeatable biological analysis results for DIA data; a novel enrichment strategy implemented in obaDIA may reduce false positive and increase the interpretability of results. Taken together, ObaDIA contributes to the standard biological analysis of proteomic data. Availability: The obaDIA is freely available from https://github.com/yjthu/obaDIA.git.

中文翻译：

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obaDIA：一步法生物学分析流程，用于独立于数据的采集和其他定量蛋白质组学数据

动机：基于DIA的蛋白质组学数据的下游生物学分析，包括蛋白质丰度统计，差异表达，功能注释和各种数据库的富集分析，是蛋白质组学研究的关键部分，但是很少有可用的集成工具和解决方案，这导致了复杂的分析过程和无法再现的分析结果。结果：我们开发了obaDIA，这是一个用于DIA数据进行生物学分析的集成式一步式管线。ObaDIA是一种快速工具，可为DIA数据生成高度可视化，全面且可重复的生物学分析结果。在obaDIA中实施的一种新颖的富集策略可以减少假阳性并增加结果的可解释性。两者合计，ObaDIA有助于蛋白质组学数据的标准生物学分析。可用性：