There is an unmet demand for microfluidics in biomedicine. We describe contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, 3-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged micro-jet of FC40 is projected through FC40 and media on to the bottom of a dish, where it washes media away to leave liquid fluorocarbon walls pinned to the substrate by interfacial forces. Such fluid walls can be built into almost any imaginable 2D circuit in minutes, which we exploit to clone cells using limiting dilution in a way that beats the Poisson limit, sub-culture adherent cells, and feed arrays of cells continuously for a week. This general method should have wide application in biomedicine.

中文翻译：

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按需喷射印刷微流体设备

生物医学中对微流体的需求未得到满足。我们描述了仅使用分配针，注射泵，三向导线，细胞培养基和不溶混的碳氟化合物（FC40）在标准培养皿上的非接触式微流控电路制造。FC40的淹没式微喷头通过FC40和介质投射到培养皿的底部，在此处冲洗介质以使液体碳氟化合物壁通过界面力固定在基板上。这样的流体壁可以在数分钟内构建到几乎任何可想象的2D回路中，我们利用有限的稀释度来克隆细胞，从而超越了Poisson极限，继代培养的贴壁细胞以及连续一周喂入细胞阵列。这种通用方法应在生物医学中有广泛的应用。