A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.,Nature Biotechnology

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A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2020-06-01 , DOI: 10.1038/s41587-020-0535-y
Julian Grünewald _{1,

2,

3} , Ronghao Zhou _{1,

2} , Caleb A Lareau _{1,

4} , Sara P Garcia ₁ , Sowmya Iyer ₁ , Bret R Miller _{1,

2} , Lukas M Langner _{1,

2} , Jonathan Y Hsu _{1,

2,

5} , Martin J Aryee _{1,

2,

3,

4} , J Keith Joung _{1,

2,

3}

Affiliation

Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

中文翻译：

双重脱氨酶CRISPR基础编辑器可同时进行腺嘌呤和胞嘧啶编辑。

现有的腺嘌呤和胞嘧啶碱基编辑器仅诱导单一类型的修饰，从而限制了可产生的DNA改变的范围。在这里，我们描述了一种基于CRISPR–Cas9的同步可编程腺嘌呤和胞嘧啶编辑器（SPACE），该编辑器可以同时引入A-to-G和C-to-T替代，同时进行最少的RNA脱靶编辑。SPACE扩大了可能的DNA序列改变的范围，扩大了CRISPR基础编辑器的研究应用。

更新日期：2020-06-01

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