ABSTRACT

Flooding is an important limiting factor for plant growth and development. Recent studies have shown that endophytic fungi symbiotic with extreme environmental plants can help hosts resisting various stresses. Previously, a styrene antioxidant NFA (Z-N-(4-hydroxystyryl) formamide) in riparian endophytic Aspergillus fumigatus was reported to effectively improve drought resistance in rice. To evaluate NFA’s ability to resist flooding of plants, we tested the antioxidant activity in yeasts, and investigated the flooding tolerance of Arabidopsis seedlings through fully submergence. The results showed that NFA could effectively alleviated oxidative damage by H₂O₂ in yeast and improved the flooding resistance of Arabidopsis. The tendency of oxidative parameters responded to flooding can be reversed by NFA, such as malondialdehyde, superoxide dismutase, ethanol dehydrogenase and NADPH oxidase. Expression analyses of NADPH oxidases and ROP protein families indicated that Atrboh D, Atrboh F and AtROP 11 may be involved in NFA-mediated flooding resistance.

摘要

洪水是限制植物生长发育的重要因素。最近的研究表明，与极端环境植物共生的内生真菌可以帮助宿主抵抗各种压力。以前，据报道，河岸内生烟曲霉中的苯乙烯抗氧化剂NFA（ZN-（4-羟基苯乙烯基）甲酰胺）可有效提高水稻的抗旱性。为了评估NFA抵抗植物泛水的能力，我们测试了酵母中的抗氧化剂活性，并通过完全浸没研究了拟南芥幼苗的泛水耐受性。结果表明，NFA可以有效减轻酵母中H ₂ O _2的氧化损伤，提高拟南芥的抗淹性。。NFA可以逆转水淹引起的氧化参数趋势，例如丙二醛，超氧化物歧化酶，乙醇脱氢酶和NADPH氧化酶。NADPH氧化酶和ROP蛋白家族的表达分析表明Atrboh D，Atrboh F和AtROP 11可能参与了NFA介导的抗洪能力。