As the final and critical step in in vitro fertilization (IVF), embryo transfer has always received much attention and deserves continuous optimization. In the present study, to explore the role of autocrine factors in embryo self-spent culture media, we prospectively compared embryo transfer with self-spent culture medium and fresh medium on clinical pregnancy outcomes. A total of 318 fresh IVF/intracytoplasmic sperm injection (ICSI) cycles were randomly allocated into two subgroups based on their transfer media (using a self-spent culture medium or new pre-equilibrated culture media), and the clinical outcomes were compared between groups. The implantation rates, clinical pregnancy rates and live birth rates for transfer using self-spent medium instead of new pre-equilibrated culture medium were slightly improved without statistical significance. Interestingly, however, biochemical pregnancy rate was found to be significantly decreased after transfer using self-spent medium for Day 3 embryos compared with new pre-equilibrated culture media. In short, embryo transfer with self-spent culture medium has shown some advantages, and large sample size studies are still needed to confirm these observations.

Abbreviations

ART: assisted reproductive technologies; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; ET: embryo transfer

中文翻译：

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用自用培养基和新鲜培养基移植的胚胎对生殖结果的比较：一项前瞻性随机试验。

因为在最后和关键的步骤在 体外受精（IVF）、胚胎移植一直备受关注，值得不断优化。在本研究中，为了探讨自分泌因子在胚胎自用培养基中的作用，我们前瞻性地比较了胚胎移植与自用培养基和新鲜培养基对临床妊娠结局的影响。将总共​​ 318 个新鲜 IVF/卵胞浆内单精子注射 (ICSI) 周期根据其转移培养基（使用自用培养基或新的预平衡培养基）随机分为两个亚组，并比较各组之间的临床结果. 使用自用培养基代替新的预平衡培养基移植的着床率、临床妊娠率和活产率略有提高，但无统计学意义。然而有趣的是，与新的预平衡培养基相比，使用自用培养基进行第 3 天胚胎移植后，生化妊娠率显着降低。简而言之，使用自用培养基进行胚胎移植显示出一些优势，仍然需要大样本研究来证实这些观察结果。

缩写

ART：辅助生殖技术；ICSI：胞浆内单精子注射；试管婴儿：体外受精；ET：胚胎移植