Abstract Context: Dicranopteris linearis L. (Gleicheniaceae) leaves have been reported to exert hepatoprotective activity. Objective: The hepatoprotective and antioxidant effects of ethyl acetate partition of D. linearis (EADL) are investigated. Materials and methods: EADL was subjected to antioxidant and anti-inflammatory studies, and phytochemical analyses. In vivo study involved six groups (n = 6) of overnight fasted Sprague Dawley rats. The test solutions [10% DMSO (normal), 10% DMSO (negative), 200 mg/kg silymarin (positive) or EADL (50, 250 or 500 mg/kg)] were administered orally once daily for 7 consecutive days followed by oral vehicle (only for normal) or hepatotoxic induction using 3 g/kg paracetamol (PCM). Results: EADL exerted ≈ 90% radical scavenging effects based on the DPPH and superoxide anion radical scavenging assays, high antioxidant capacity in the oxygen radical absorbance capacity assay (≈ 555,000 units), high total phenolic content (≈ 350 mg GAE/100 g extract) (p < 0.05), but low anti-inflammatory effect. EADL also attenuated PCM-induced liver intoxication as indicated by reduced level of serum liver enzymes; increased activity of endogenous enzymatic antioxidant (superoxide dismutase – 8.3 vs. 4.0 U/g tissue; catalase – 119 vs. 52 U/g tissue) and; reduced level of lipid peroxidation marker (2.7 vs. 5.0 µM). Preliminary screening of EADL revealed the presence of saponins, tannins and flavonoids with further HPLC analysis demonstrating the presence of rutin and quercetin. Discussion and conclusion: EADL exerted hepatoprotective and antioxidant activities; thus, these data support the potential use of D. linearis as a new source for future hepatoprotective drug development.

中文翻译：

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Dicranopteris linearis 叶提取物对扑热息痛诱导的大鼠肝中毒的保肝和抗氧化活性

摘要背景：据报道，Dicranopteris linearis L. (Gleicheniaceae) 的叶子具有保肝活性。目的：研究了乙酸乙酯分配的线虫（EADL）的保肝和抗氧化作用。材料和方法：对 EADL 进行抗氧化和抗炎研究以及植物化学分析。体内研究涉及六组 (n = 6) 隔夜禁食的 Sprague Dawley 大鼠。测试溶液 [10% DMSO（正常）、10% DMSO（阴性）、200 mg/kg 水飞蓟素（阳性）或 EADL（50、250 或 500 mg/kg）] 每天口服一次，连续 7 天，然后口服载体（仅用于正常）或使用 3 g/kg 扑热息痛 (PCM) 诱导肝毒性。结果：基于 DPPH 和超氧阴离子自由基清除试验，EADL 发挥 ≈ 90% 自由基清除作用，在氧自由基吸收能力测定中具有高抗氧化能力（≈ 555,000 单位），总酚含量高（≈ 350 mg GAE/100 g 提取物）（p < 0.05），但抗炎作用低。EADL 还减轻了 PCM 诱导的肝中毒，表现为血清肝酶水平降低；内源性酶促抗氧化剂的活性增加（超氧化物歧化酶 – 8.3 与 4.0 U/g 组织；过氧化氢酶 – 119 与 52 U/g 组织）和；脂质过氧化标记物水平降低（2.7 与 5.0 µM）。EADL 的初步筛选显示存在皂苷、单宁和黄酮类化合物，进一步的 HPLC 分析表明存在芦丁和槲皮素。讨论与结论：EADL具有保肝和抗氧化活性；因此，这些数据支持 D 的潜在用途。