Abstract

In this study, the complete mitochondrial genome of the leafhopper species Empoascanara dwalata (Hemiptera: Cicadellidae: Typhlocybinae) was sequenced and annotated, and the sequence is available in the GenBank with accession number: MT350235. The circular mitogenome with 15,271 bp in length, including 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and one AT-rich region. The nucleotide composition is as follows: A (41.7%), T (34.7%), G (10.6%), and C (13.0%). 11 PCGs have ATN as the start codon, except for atp8 and nad5 genes have TTG. The conventional termination codons (TAA or TAG) occur in 11 PCGs, while cox2 and nad5 use incomplete codon (T––) as termination codon. Phylogenetic analysis using 13 PCGs showed that E. dwalata was clustered with three other Typhlocybine species, which was consistent with the conventional classification.

摘要

在这项研究中，对叶蝉物种Empoascanara dwalata（半翅目：Cicadellidae：Typholocybinae）的完整线粒体基因组进行了测序和注释，该序列可在GenBank中获得，登录号：MT350235。环状有丝分裂基因组，长15271 bp，包括13个蛋白质编码基因（PCG），22个tRNA基因，2个rRNA基因和1个富含AT的区域。核苷酸组成如下：A（41.7％），T（34.7％），G（10.6％）和C（13.0％）。除atp8和nad5基因具有TTG外，有11个PCG具有ATN作为起始密码子。常规终止密码子（TAA或TAG）出现在11个PCG中，而cox2和nad5使用不完整的密码子（T––）作为终止密码子。用13种PCG进行的系统发育分析表明，dwalata大肠埃希菌与其他三种Typhlocybine种聚在一起，这与常规分类是一致的。