ABSTRACT Oocystis-like algae are diverse and found throughout the world. In this study, two new strains of algae with colonies resembling Oocystis were identified and successfully cultured in the laboratory. A polyphasic approach, including light microscopy, transmission electron microscopy and phylogenetic analyses of 18S rDNA and rbcL cpDNA, was used to characterize the strains. Observation of the two strains showed a regular colonial morphology (cells/daughter colony are parallel to each other and positioned near the edge of the mother cell wall) and a characteristic fibrillar network structure on the mother cell wall is obvious in light microscopy, which distinguished them from other Oocystis-like members described previously. Molecular analyses revealed that the two strains form an independent lineage within the subfamily Makinoelloideae (Oocystaceae, Trebouxiophyceae), separated from other genera. Therefore, we described this alga as Reticulocystis yunnanensis gen. et sp. nov. The genera of Makinoelloideae with similar colonial morphotypes were revealed as not monophyletic and the relationships between them remained undetermined. Additional taxon sampling and data from additional multiple genes are necessary to assess the taxonomy of the Makinoelloideae.

中文翻译：

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云南网孢子菌属 等 sp. 11月，淡水卵囊藻（Trebouxiophyceae，Chlorophyta）的新成员

摘要 类卵囊藻种类繁多，遍布世界各地。在这项研究中，鉴定出两种具有类似卵囊藻的菌落的新藻类菌株，并在实验室中成功培养。使用多相方法来表征菌株，包括光学显微镜、透射电子显微镜和 18S rDNA 和 rbcL cpDNA 的系统发育分析。观察两株菌落形态规则（细胞/子代菌落相互平行，靠近母细胞壁边缘），光镜下可见母细胞壁特征性的纤维网状结构，区分它们来自之前描述的其他类似卵囊的成员。分子分析表明，这两个菌株在 Makinoelloideae 亚科（卵囊科，Trebouxiophyceae）内形成一个独立的谱系，与其他属分开。因此，我们将这种藻类描述为云南网纹藻属（Reticulocystis yunnanensis gen）。等 sp. 十一月 具有相似群落形态类型的牧牛科属被揭示为不是单系的，它们之间的关系仍未确定。需要额外的分类单元采样和来自额外多个基因的数据来评估 Makinoelloideae 的分类。