ABSTRACT Haslea ostrearia (Gaillon) Simonsen is famous among diatoms for producing a blue pigment. Genome sequencing of different strains of H. ostrearia led to the discovery of the complete genomes of two new bacilladnaviruses, HOV-148 and HOV-235. The DNA sequences of these viruses were identified in H. ostrearia strains that had been maintained for several years in a culture collection. Some of these strains are the products of in vitro episodes of auxosporulation and thus have never been in contact with their natural biotope. At 4567 and 4538 bp, the HOV-148 and HOV-235 genomes are shorter than those of most diatom-infecting viruses known to date. They each contain four open reading frames and display highest similarities with sequences from viruses found in a gastropod from New Zealand, a proximity confirmed by phylogenetic analysis. HOV-148 and HOV-235 were identified in three clones of H. ostrearia that were collected in oyster ponds from distinct locations in the Bay of Bourgneuf (France). Given that our search for similar viral sequences in genomic data derived from other sub-populations or species of the genus Haslea throughout the world was unsuccessful, we hypothesize that these viruses could have a link with the peculiar biotope of the oyster ponds.

中文翻译：

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与硅藻 Haslea ostrearia 相关的两种新芽孢杆菌病毒

摘要 Haslea ostrearia (Gaillon) Simonsen 在硅藻中因生产蓝色颜料而闻名。H. ostrearia 不同菌株的基因组测序导致发现了两种新的杆状病毒 HOV-148 和 HOV-235 的完整基因组。这些病毒的 DNA 序列是在培养物保藏中心保存多年的 H. ostrearia 菌株中鉴定的。其中一些菌株是体外辅助孢子形成的产物，因此从未接触过它们的天然生物群落。在 4567 和 4538 bp 处，HOV-148 和 HOV-235 基因组比迄今为止已知的大多数硅藻感染病毒的基因组短。它们每个都包含四个开放阅读框，并与在新西兰腹足动物中发现的病毒序列显示出最高的相似性，系统发育分析证实了这种接近性。HOV-148 和 HOV-235 在三个 H. ostrearia 克隆中被鉴定出来，这些克隆是从 Bourgneuf 湾（法国）不同地点的牡蛎池塘中收集的。鉴于我们在来自世界各地 Haslea 属的其他亚群或物种的基因组数据中寻找类似的病毒序列未成功，我们假设这些病毒可能与牡蛎池塘的特殊生物群落有关。