Abstract

Several studies have demonstrated that peptides obtained from the proteins of different bean species have the potential to act on therapeutic targets of noncommunicable chronic diseases or NCDs. However, peptides with great structural diversity can be obtained from the hydrolysis of proteins present in foods. Therefore, the present review had the objective of identifying, in silico, the possibility of obtaining peptides with potential biological activity from the storage globulin proteins of the bean species Phaseolus vulgaris (L.), Vigna angularis (Willd.), Vigna radiata (L.) and Vigna unguiculata (L.) Walp., using the UniProtKB, BIOPEP and PeptideRanker databases, as well as reviewing available research reports that showed evidence bioactive properties of peptides obtained from beans via in vitro assays. For all the species studied, the highest frequency of the occurrence of bioactive fragments was found for the inhibition of dipeptidyl peptidase-IV, followed by the inhibition of the angiotensin-converting enzyme and by antioxidant activity. The inhibition of the two enzymes is the therapeutic target of drugs used for type 2 diabetes mellitus (T2DM) and for hypertension, respectively, while the antioxidant activity can prevent the development of several chronic diseases related to oxidative stress.

摘要

几项研究表明，从不同豆类的蛋白质中提取的肽有可能作用于非传染性慢性病或 NCD 的治疗靶点。然而，可以从食物中存在的蛋白质的水解中获得具有极大结构多样性的肽。因此，本审查过的目标识别，的在计算机芯片上，从bean物种的存储球蛋白获得具有潜在的生物活性的肽的可能性菜豆（L.），小豆（斑鸠菊。），绿豆（L .) 和豇豆(L.) Walp.，使用 UniProtKB、BIOPEP 和 PeptideRanker 数据库，并审查了现有的研究报告，这些报告显示了通过体外试验从豆类中获得的肽的生物活性特性。对于所有研究的物种，发现生物活性片段出现频率最高的是二肽基肽酶-IV 的抑制，其次是血管紧张素转换酶的抑制和抗氧化活性。抑制这两种酶分别是用于治疗 2 型糖尿病 (T2DM) 和高血压的药物的治疗靶点，而抗氧化活性可以预防几种与氧化应激相关的慢性疾病的发展。