In our study, we monitored the inactivation of two important viruses that are critical in animal husbandry throughout the world. To evaluate the influence of the composting process on inactivation of avian influenza virus (H9N2) in poultry manure compost (PMC) and Encephalomyocarditis virus (EMCV) in pig (swine) manure compost (SMC), the H9N2 and EMCV were injected in dialysis cassettes and buried in two different manure compost piles of poultry and pig manure, respectively. The highest temperature achieved in the PMC and SMC piles during the test period were 75 °C and 73.5 °C, respectively. At the completion of the composting for 168 h, inactivation effect appeared to be more sensitive in H9N2 than EMCV. The vitality of H9N2 decreased by 6.25±0.35 log₁₀TCID50/mL to 0.0 log₁₀TCID50/mL within 1 h of the composting. The vitality of EMCV decreased from 7.75±0.35 log₁₀TCID50/mL to 1.50 log₁₀TCID50/mL within 24 h of starting the composting process. However, the activation of EMCV was not decreased (from 7.75±0.35 to 7.50±0.71 log10TCID50/mL) in the control treatment (not inserted in composts) after 168h, while the activation of H9N2 in dialysis cassettes was significantly decreased (from 6.25±0.35 log₁₀TCID50/mL to 2.00±0.6 log₁₀TCID50/mL). Our study demonstrated the effectiveness of the composting treatment in inactivating the viruses studied, which was not the case with air treatment.

中文翻译：

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堆肥降低了家禽粪便中H9N2的活力，降低了猪粪中EMCV的含量，从而使这些动物粪便得到了环境友好的利用：一项初步研究。

在我们的研究中，我们监测了在全世界畜牧业中至关重要的两种重要病毒的灭活。为了评估堆肥过程对禽粪堆肥（PMC）禽流感病毒（H9N2）和猪（猪）粪堆肥（SMC）的脑心肌炎病毒（EMCV）灭活的影响，将H9N2和EMCV注入透析盒中并分别埋在两个不同的家禽和猪粪堆肥中。在测试期间，PMC和SMC堆中达到的最高温度分别为75°C和73.5°C。在堆肥完成168小时后，H9N2的灭活作用似乎比EMCV更敏感。H9N2的活力下降了6.25±0.35 log ₁₀ TCID50 / mL，降至0.0 log ₁₀堆肥后1小时内的TCID50 / mL。在堆肥过程开始24小时内，EMCV的活力从7.75±0.35 log ₁₀ TCID50 / mL降低到1.50 log ₁₀ TCID50 / mL。然而，对照治疗（未插入堆肥中）在168h之后，EMCV的活化并未降低（从7.75±0.35降至7.50±0.71 log10TCID50 / mL），而透析盒中H9N2的活化却显着降低（从6.25± 0.35 log ₁₀ TCID50 / mL至2.00±0.6 log ₁₀ TCID50 / mL）。我们的研究证明了堆肥处理在灭活所研究病毒方面的有效性，而空气处理则并非如此。