One major facilitator superfamily transporter is responsible for propionic acid tolerance in Pseudomonas putida KT2440,Microbial Biotechnology

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One major facilitator superfamily transporter is responsible for propionic acid tolerance in Pseudomonas putida KT2440
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-05-31 , DOI: 10.1111/1751-7915.13597
Chao Ma _{1,

2,

3} , Qingxuan Mu _{1,

3} , Yubin Xue _{1,

3} , Yanfen Xue ₂ , Bo Yu ₁ , Yanhe Ma ₂

Affiliation

Propionic acid (PA) has been widely used as a food preservative and chemical intermediate in the agricultural and pharmaceutical industries. Environmental and friendly biotechnological production of PA from biomass has been considered as an alternative to the traditional petrochemical route. However, because PA is a strong inhibitor of cell growth, the biotechnological host should be not only able to produce the compound but the host should be robust. In this study, we identified key PA tolerance factors in Pseudomonas putida KT2440 strain by comparative transcriptional analysis in the presence or absence of PA stress. The identified major facilitator superfamily (MFS) transporter gene cluster of PP_1271, PP_1272 and PP_1273 was experimentally verified to be involved in PA tolerance in P. putida strains. Overexpression of this cluster improved tolerance to PA in a PA producing strain, what is useful to further engineer this robust platform not only for PA synthesis but for the production of other weak acids.

中文翻译：

一种主要的促进者超家族转运蛋白是恶臭假单胞菌KT2440中丙酸耐受性的原因。

丙酸（PA）已被广泛用作农业和制药行业的食品防腐剂和化学中间体。从生物质中环保和友好的生物技术生产PA已被认为是传统石化路线的替代方案。但是，由于PA是细胞生长的强抑制剂，因此生物技术宿主不仅应该能够生产该化合物，而且宿主也应该坚固。在这项研究中，我们通过存在或不存在PA应激的比较转录分析确定了恶臭假单胞菌KT2440菌株中关键的PA耐受因子。通过实验验证，已鉴定出的PP_1271，PP_1272和PP_1273主要促进者超家族（MFS）转运蛋白基因簇与恶臭假单胞菌的PA耐受性有关株。该簇的过表达改善了在PA产生菌株中对PA的耐受性，这对于进一步工程化该鲁棒的平台不仅对于PA合成而且对于其他弱酸的产生都是有用的。

更新日期：2020-05-31

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