A revolutionary avenue for vibrational imaging with super‐multiplexing capability can be seen in the recent development of Raman‐active bioortogonal tags or labels. These tags and isotopic labels represent groups of chemically inert and small modifications, which can be introduced to any biomolecule of interest and then supplied to single cells or entire organisms. Recent developments in the field of spontaneous Raman spectroscopy and stimulated Raman spectroscopy in combination with targeted imaging of biomolecules within living systems are the main focus of this review. After having introduced common strategies for bioorthogonal labeling, we present applications thereof for profiling of resistance patterns in bacterial cells, investigations of pharmaceutical drug‐cell interactions in eukaryotic cells and cancer diagnosis in whole tissue samples. Ultimately, this approach proves to be a flexible and robust tool for in vivo imaging on several length scales and provides comparable information as fluorescence‐based imaging without the need of bulky fluorescent tags.

中文翻译：

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成像用于细胞和组织成像的不可见生物正交拉曼探针。

具有超多路复用功能的振动成像的革命性途径可以在拉曼活性生物正交标签或标签的最新发展中看到。这些标签和同位素标记代表化学惰性和微小修饰的组，可以将其引入任何目标生物分子中，然后提供给单个细胞或整个生物体。自发拉曼光谱和受激拉曼光谱与活体系统内生物分子的靶向成像相结合的最新进展是本综述的主要重点。在介绍了生物正交标记的常用策略之后，我们将介绍其在细菌细胞中耐药模式图谱分析，真核细胞中药物药-细胞相互作用的研究以及整个组织样品中癌症诊断的应用。