Piscine orthoreovirus genotype 1 (PRV‐1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and trout. Here, we report the results of laboratory challenges using juvenile Chinook salmon, coho salmon and rainbow trout injected with tissue homogenates from Atlantic salmon testing positive for PRV‐1 or with control material. Fish were sampled at intervals to assess viral RNA transcript levels, haematocrit, erythrocytic inclusions and histopathology. While PRV‐1 replicated in all species, there was negligible mortality in any group. We observed a few erythrocytic inclusion bodies in fish from the PRV‐1‐infected groups. At a few time points, haematocrits were significantly lower in the PRV‐1‐infected groups relative to controls, but in no case was anaemia noted. The most common histopathological finding was mild, focal myocarditis in both the non‐infected controls and PRV‐1‐infected fish. All cardiac lesions were judged mild, and none were consistent with those of HSMI. Together, these results suggest all three species are susceptible to PRV‐1 infection, but in no case did infection cause notable disease in these experiments.

中文翻译：

---

奇努克鲑鱼（Oncorhynchus tshawytscha），银大麻哈鱼（O. kisutch）和虹鳟鱼（O. mykiss）感染正畸形正畸病毒基因型1（PRV-1）的后果。

猪 正咽病毒基因型1（PRV-1）是养殖大西洋鲑鱼（Salmo salar）的心脏和骨骼肌炎症（HSMI）的病原体L.）。在北美西部的太平洋鲑鱼中也发现了这种病毒，这引起了人们对本土鲑鱼和鳟鱼风险的担忧。在这里，我们报告了使用奇努克鲑，银大麻哈鱼和虹鳟鱼注入大西洋鲑鱼组织匀浆对PRV-1呈阳性或对照材料注射时的实验室挑战结果。每隔一段时间对鱼取样，以评估病毒RNA转录水平，血细胞比容，红细胞包涵体和组织病理学。虽然PRV-1在所有物种中都有复制，但任何一组的死亡率都可以忽略不计。我们在PRV-1感染组的鱼中观察到一些红细胞包涵体。在几个时间点上，PRV-1感染组的血触风险明显低于对照组，但没有发现贫血。最常见的组织病理学发现是轻度的，未感染的对照组和PRV-1感染的鱼均患有局灶性心肌炎。所有心脏病变均被判定为轻度病变，且均与HSMI一致。总之，这些结果表明，这三种物种均容易感染PRV-1，但在这些实验中，在任何情况下感染都不会引起明显的疾病。