RNA interference (RNAi) targeting lethal genes in insects has great potential for sustainable crop protection. Compared with traditional double‐stranded (ds)RNA delivery systems, nanoparticles such as chitosan, liposomes, and cationic dendrimers offer advantages in delivering dsRNA/small interfering (si)RNA to improve RNAi efficiency, thus promoting the development and practice of RNAi‐based pest management strategies. Here, we illustrate the limitations of traditional dsRNA delivery systems, reveal the mechanism of nanoparticle‐mediated RNAi, summarize the recent progress and successful applications of nanoparticle‐mediated RNAi in pest management, and finally address the prospects of nanoparticle‐based RNA pesticides.

中文翻译：

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纳米粒子介导的双链 RNA 递送系统：一种有前景的可持续害虫管理方法

针对昆虫致死基因的 RNA 干扰 (RNAi) 在可持续作物保护方面具有巨大潜力。与传统的双链 (ds)RNA 递送系统相比，壳聚糖、脂质体和阳离子树枝状大分子等纳米粒子在递送 dsRNA/小干扰 (si)RNA 以提高 RNAi 效率方面具有优势，从而促进了基于 RNAi 的开发和实践。害虫管理策略。在这里，我们阐述了传统 dsRNA 递送系统的局限性，揭示了纳米粒子介导的 RNAi 的机制，总结了纳米粒子介导的 RNAi 在害虫管理中的最新进展和成功应用，最后阐述了基于纳米粒子的 RNA 杀虫剂的前景。