Mirabilis crinkle mosaic virus (MiCMV) was tentatively classified as a new member of the genus Potyvirus in the family Potyviridae in 2019. However, it was considered to be basella rugose mosaic virus based on the sequence similarity of the coat protein (CP) region. In this study, infectious MiCMV cDNA clones under the control of the 35S promoter were constructed with an In-Fusion cloning method. Systemically infected leaves of Mirabilis jalapa and Nicotiana benthamiana plants inoculated with pMiCMV and pMiCMV-NIb/eGFP had mosaic symptoms by 5 dpi. Infections were confirmed by a western blot analysis, electron microscopy, RT-PCR, and the inoculation of N. benthamiana seedlings with progeny virions. Systemic infections were not observed after Nicotiana glutinosa leaves were similarly inoculated, with eGFP fluorescence detected only in the inoculated leaves. Interestingly, the symptoms induced by pMiCMV and pMiCMV-NIb/eGFP were not similar to those caused by the wild-type MiCMV in Basella rubra plants. Moreover, RT-PCR analyses of B. rubra plants with virus-specific primers (MicpF and MicpR) indicated that a non-target fragment corresponding to the MiCMV CP coding region was amplified. This is the first report of the construction of a biologically active, full-length cDNA copy of the MiCMV RNA genome.

紫茉莉起皱花叶病毒（MiCMV）暂定分类为属的一个新成员-马铃薯Y病毒在家庭马铃薯Y病毒在2019年但是，它被认为是有皱纹的落葵镶嵌基于所述外壳蛋白（CP）区域的序列相似性病毒。在这项研究中，采用融合克隆方法构建了在35S启动子控制下的感染性MiCMV cDNA克隆。全身感染叶紫茉莉和氏烟用pMiCMV和pMiCMV - NIB / eGFP的接种植物5 DPI有马赛克的症状。通过蛋白质印迹分析，电子显微镜，RT-PCR和接种本氏烟草证实证实感染。子代病毒体的幼苗。相似地接种烟草叶片后，未观察到全身感染，仅在接种叶片中检测到eGFP荧光。有趣的是，在Basella rubra植物中，pMiCMV和pMiCMV-NIb / eGFP诱导的症状与野生型MiCMV引起的症状不同。此外，RT-PCR分析的B.杨梅植物与病毒特异性引物（MicpF和MicpR）指示对应于MiCMV CP编码区的非靶片段被扩增。这是MiCMV RNA基因组具有生物学活性的全长cDNA拷贝的首次报道。