Abstract Direct protein purification from raw materials by chromatographic media represents an enormous challenge, especially when particulate solids and high charge solute are present. In this sense, protein purification from cheese whey is an excellent opportunity to test new chromatographic matrices that can be applied to direct protein isolation. The present work shows the utility of novel multimodal chitosan-based chromatographic matrices for obtaining lactoferrin (LF) and a whey protein isolate (WPI) directly from cheese whey without any pretreatment. A central composite experimental design was used to optimise an operative sequence. This sequence involved LF capture using sulfanilic acid-modified chitosan mini-spheres followed by the capture of the massive remnant proteins using glycidyl trimethylammonium-modified chitosan mini-spheres. Interestingly, a yield of 68% and 70% purity degree was obtained for LF, and 2.71 mg of WPI mL−1 whey was obtained in the WPI recovery process, revealing a potential industrial use of the developed matrices and processes.

中文翻译：

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使用壳聚糖微型球从奶酪乳清中纯化乳铁蛋白和分离乳清蛋白

摘要 通过色谱介质从原材料中直接纯化蛋白质是一项巨大的挑战，尤其是当存在颗粒状固体和高电荷溶质时。从这个意义上说，从奶酪乳清中纯化蛋白质是测试可用于直接蛋白质分离的新色谱基质的绝佳机会。目前的工作展示了新型多峰壳聚糖色谱基质在无需任何预处理的情况下直接从奶酪乳清中获得乳铁蛋白 (LF) 和乳清蛋白分离物 (WPI) 的效用。中央复合实验设计用于优化手术顺序。该序列涉及使用对氨基苯磺酸修饰的壳聚糖微型球体进行 LF 捕获，然后使用缩水甘油基三甲基铵修饰的壳聚糖微型球体捕获大量残余蛋白质。