Abstract Zinc (Zn) is an important higher plant micronutrient; however, high amounts of Zn in soil may negatively affect plant growth. In this study, two wheat (Triticum aestivum L.) genotypes [Aikang58 (AK) and Zhengmai0856 (ZM)] were grown in nutrient solutions containing various Zn concentrations and were characterized using physiological and molecular approaches. Compared to sufficient Zn supply (0.25 mg L−1), excess Zn (2.5 mg L−1) significantly decreased the biomass and root morphological features (root length, volume, and surface area) of AK, whereas only a slight decrease was observed in ZM. ZM maintained higher Zn concentration and translocation factor, and lower net photosynthetic rate than AK under excess Zn supply. Excess Zn decreased Cu, Fe, and Mn concentrations in roots of both wheat genotypes, whereas Mn concentrations in shoots increased. In addition, the expression of TaZIPs indicated that TaZIP5, −6, and −13 in roots are primarily involved in Zn uptake when the medium has elevated Zn levels. The translocation of more Zn from the roots to shoots facilitates in maintaining the efficiency of photosynthesis by using lower amounts of photosynthetic pigments, and TaZIP genes involved in cell ion homeostasis contributes to greater Zn tolerance in ZM than AK.

中文翻译：

---

标题：过量锌诱导的两种小麦基因型中 TaZips 生理参数和表达水平的变化

摘要 锌（Zn）是一种重要的高等植物微量元素；然而，土壤中大量的锌可能会对植物生长产生负面影响。在这项研究中，两种小麦 (Triticum aestivum L.) 基因型 [Aikang58 (AK) 和 Zhengmai0856 (ZM)] 在含有不同锌浓度的营养液中生长，并使用生理学和分子学方法进行表征。与充足的 Zn 供应（0.25 mg L-1）相比，过量的 Zn（2.5 mg L-1）显着降低了 AK 的生物量和根系形态特征（根长、体积和表面积），而仅观察到轻微下降在 ZM。在过量的锌供应下，ZM 比 AK 保持更高的 Zn 浓度和易位因子，以及更低的净光合速率。过量的锌降低了两种小麦基因型根中铜、铁和锰的浓度，而芽中锰的浓度增加。此外，TaZIPs 的表达表明，当培养基中锌含量升高时，根中的 TaZIP5、-6 和 -13 主要参与锌的吸收。更多的锌从根部转移到枝条，通过使用较少量的光合色素促进了光合作用的效率，而参与细胞离子稳态的 TaZIP 基因有助于 ZM 中比 AK 更大的锌耐受性。