Abstract Hydrogen gas (H2) is known for its capability to alleviate detrimental effects of salinity in plants. However, the underlying physiological and molecular mechanisms behind this phenomenon remain poorly understood. In this study, we investigated the mechanistic basis of H2 amelioration of the salt stress in barley (Hordeum vulgare). NaCl addition induced significant inhibition of the root elongation and resulted in a loss of the cell viability; these detrimental effects were substantially reversed by treatment with hydrogen reach water (HRW). Electrophysiological experiments using non-invasive ion flux measuring MIFE technique revealed that the beneficial effects of HRW on salinity tolerance could be explained by (1) higher rate of Na+ extrusion from roots mediated by SOS1-like Na+/H+ exchanger in the root epidermis, and (2) better root K+ retention resulting from ability of HRW-treated plants to prevent NaCl-induced membrane depolarization and reduced sensitivity of K+ efflux channels to ROS. Taken together, these two factors resulted in more favorable Na/K ratio and explained beneficial effects of HRW on salinity tolerance in barley.

中文翻译：

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了解富氢水改善大麦耐盐性的机理基础

摘要 氢气 (H2) 因其能够减轻盐分对植物的不利影响而闻名。然而，这种现象背后的潜在生理和分子机制仍然知之甚少。在这项研究中，我们研究了 H2 改善大麦（Hordeum vulgare）盐胁迫的机制基础。添加NaCl显着抑制根伸长并导致细胞活力丧失；通过使用氢到达水 (HRW) 进行处理，这些不利影响得到了显着逆转。使用非侵入性离子通量测量 MIFE 技术的电生理实验表明，HRW 对耐盐性的有益影响可以解释为 (1) 根表皮中 SOS1 样 Na+/H+ 交换剂介导的根部 Na+ 排出率较高，(2) 由于 HRW 处理的植物能够防止 NaCl 诱导的膜去极化和降低 K+ 流出通道对 ROS 的敏感性，因此根系 K+ 保留更好。综上所述，这两个因素导致了更有利的 Na/K 比，并解释了 HRW 对大麦耐盐性的有益影响。