Objectives

Different approaches are currently undertaken to structure the endosseous part of zirconia implants. The purpose of the present study was to evaluate how surface roughness and monoclinic to tetragonal phase ratio of zirconia affect cell behavior of human osteoblasts.

Methods

Zirconia discs with five different surface structures were produced: machined; machined heat-treated; polished; polished heat-treated; sandblasted, etched and heat-treated (cer.face 14, vitaclinical). The specimen surfaces were then characterized in terms of monoclinic to tetragonal phase ratio, wettability, roughness and visualized using scanning electron microscopy. To determine the reaction of the human osteoblastic cells (MG-63) to the surface roughness and monoclinic to tetragonal phase ratio of zirconia, cell spreading, morphology, actin cytoskeleton, viability and gene expression of alkaline phosphatase (ALP), collagen type I (COL) and osteocalcin (OCN) were assessed.

Results

Heat-treatment of the specimens significantly improved the surface wettability. With increased surface roughness Ra of the specimens, cell spreading was reduced. Cell viability after 24 h correlated linearly with the tetragonal phase ratio of the specimens. Gene expression after 24 h and 3 d was comparable on all specimens irrespective their surface roughness or monoclinic to tetragonal phase ratio.

Significance

Smooth zirconia surfaces with a high tetragonal phase ratio revealed best surface conditions for MG-63 osteoblastic cells and may be considered to design the endosseous part of zirconia implants.

中文翻译：

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氧化锆的晶体结构影响成骨细胞的行为。

目标

当前采用不同的方法来构造氧化锆植入物的骨内部分。本研究的目的是评估氧化锆的表面粗糙度和单斜晶与四方晶之比如何影响人类成骨细胞的细胞行为。

方法

制作了具有五种不同表面结构的氧化锆圆盘：机加工热处理；抛光 抛光热处理 喷砂，蚀刻和热处理（面14，临床）。然后根据单斜晶与四方晶之比，润湿性，粗糙度对样品表面进行表征，并使用扫描电子显微镜对其进行可视化。为了确定人类成骨细胞（MG-63）对氧化锆的表面粗糙度和单斜晶与四方晶之比的反应，细胞扩散，形态，肌动蛋白细胞骨架，碱性磷酸酶（ALP）的活力和基因表达，I型胶原（评估COL和骨钙素（OCN）。

结果

样品的热处理显着改善了表面润湿性。随着样品表面粗糙度Ra的增加，细胞扩散减少。24小时后细胞活力与样品的四方相比例线性相关。24 h和3 d后的基因表达在所有标本上均具有可比性，无论其表面粗糙度或单斜晶与四方晶之比。

意义

具有高四方相比的光滑氧化锆表面显示出MG-63成骨细胞的最佳表面条件，可以考虑设计氧化锆植入物的骨内部分。