The study investigated the effects of transplantation and reconstitution of indigenous and exogenous epiphytic microbiota on the fermentation quality and microbial community of red clover silage. Sterile red clover was inoculated with distilled water (RC0), extracted epiphytic microbiota of red clover (RC), maize (MZ), and sorghum (SG). RC inoculation rapidly decreased pH at the onset of ensiling. The LA concentration and ratio of LA/AA were higher in RC silage during entire ensiling while MZ silage during late phase of ensiling. Pediococcus was dominant in RC early silage, while Lactobacillus was abundant in MZ final silage. The SG terminal silage had higher pH (>4.50) and dominated by Sphingomonas, Enterobacter, and Novosphingobium. RC and MZ microbiota were beneficial in enhancing fermentation quality and microbial community in red clover silage. Transplantation and reconstitution of epiphytic microbiota can be a successful method to assess the effective and eco-friendly additive for the targeted crop.

该研究调查了本地和外生附生微生物的移植和重组对红三叶青贮饲料发酵质量和微生物群落的影响。用蒸馏水（RC0），红三叶草（RC），玉米（MZ）和高粱（SG）的附生微生物接种无菌红三叶草。在接种开始时，RC接种会迅速降低pH值。在整个青贮期间，RC青贮饲料中的LA浓度和LA / AA比例较高，而在青贮后期则为MZ青贮饲料。在RC早期青贮饲料中，小球菌占主导地位，而在MZ最终青贮饲料中，乳酸杆菌含量较高。SG末端青贮饲料的pH值较高（> 4.50），并以鞘氨醇单胞菌，肠杆菌和新孢子菌为主。RC和MZ菌群有利于提高红三叶草青贮饲料的发酵质量和微生物群落。附生微生物的移植和重建可能是评估目标作物有效且环保的添加剂的成功方法。