Microalgae have potential for eco-friendly chemicals, however, only a few of them have been exploited. This study investigated four new microalgae on their growth and production of fatty acids and carotenoids grown photoautotrophically, heterotrophically, or mixotrophically using improved techniques to monitor glucose metabolism, carotenoid and lipid formation. Depending on trophic modes, the microalgae demonstrated different growth rates, ranging from 0.23 d⁻¹ of photoautotrophic Selenastraceae sp. B10 to 1.03 d⁻¹ of heterotrophic Pectinodesmus sp. F13. Scenedesmus sp. DHS gained the highest biomass due to its fastest glucose consumption. Heterotrophic Selenastraceae sp. B10 accumulated the highest amounts of lutein (7.49 mg g⁻¹) and lipids (63.15% of DCW), while mixotrophic Pectinodesmus sp. F13 achieved the highest productivity of lutein (5.60 mg L⁻¹ d⁻¹). Photoautotrophic Chlorella sp. Mg exhibited the highest production of α-linolenic acid (37.19% of total fatty acids). Taken together, the studied microalgae have great potential as feedstock for commercially attractive production of lipids or/and lutein.

微藻类具有用于生态友好型化学品的潜力，但是，只有少数被利用。这项研究使用改进的技术监测葡萄糖代谢，类胡萝卜素和脂质的形成，研究了四种新的微藻对它们的光养，异养或杂养生长的脂肪酸和类胡萝卜素的生长和产量。取决于营养模式，微藻表现出不同的生长速率，范围为光合自养的硒生丝藻的0.23 d ^-1。B10至1.03 d ^-1的异养果胶菌。F13。Scenedesmus sp。由于其最快的葡萄糖消耗，DHS获得了最高的生物量。异养硒亚科sp。B10累积了最高量的叶黄素（7.49 mg g ^-1）和脂质（占DCW的63.15％），而混合营养型果蝇（Pectinodesmus sp。）。F13实现了叶黄素的最高生产率（5.60 mg L ^-1  d ^-1）。光自养小球藻藻。镁表现出最高的α-亚麻酸产量（占总脂肪酸的37.19％）。综上所述，所研究的微藻作为原料具有巨大潜力，可用于生产具有商业吸引力的脂质或/和叶黄素。