Insulin signaling pathway is an ancient and highly conserved pathway known to play critical roles in cell growth, control and metabolic regulation. In this study, we identified and characterized two insulin receptor genes (TsIR-1316 and TsIR-4810) from Taenia solium. TsIR-1316 was grouped with E. multilocularis insulin receptor (EmIR-1) and TsIR-4810 was closer to Taenia pisiformis insulin-like growth factor receptor (TpIR) on the same branch with a very high bootstrap value. TsIR-1316 was located on the integument of larvae and adult worms, as well as the ovary of adults and eggs. Alternatively, TsIR-4810 was located in the parenchyma and reproductive organs of the adult worms. By using in vitro cultivation systems with Cysticercus pisiformis as a model, we demonstrated that anti-TsIRs-LBD antibodies could effectively block the insulin signaling pathway, resulting in reduced phosphorylation of the insulin receptor as well as lower levels of glucose uptake and glycogen synthesis. The rabbits immunized with TsIR-1316-LBD, TsIR-4810-LBD and TsIR-1316-LBD + TsIR-4810-LBD produced protection against infection of T. pisiformis as demonstrated by a 94.6%, 96% and 80% reduction of establishment of larvae, respectively. These data suggested that TsIR-1316-LBD and TsIR-4810-LBD are promising vaccine candidates or novel drug targets against swine cysticercosis.

胰岛素信号传导途径是古老且高度保守的途径，已知在细胞生长，控制和代谢调节中起关键作用。在这项研究中，我们鉴定和鉴定了来自Ta虫的两个胰岛素受体基因（TsIR-1316和TsIR-4810）。TsIR-1316与多眼大肠杆菌胰岛素受体（EmIR-1）分组，而TsIR-4810在同一分支上更接近牛带状en虫胰岛素样生长因子受体（TpIR），具有非常高的自举值。TsIR-1316位于幼虫和成虫的外壳上，以及成虫和卵子的卵巢上。另外，TsIR-4810位于成虫的实质和生殖器官中。通过使用体外培养系统豆状囊尾蚴为模型，我们证实抗TsIRs-LBD抗体可有效阻断胰岛素信号传导途径，导致胰岛素受体的磷酸化降低，以及葡萄糖摄取和糖原合成的较低水平。与TSIR-1316-LBD，TSIR-4810-LBD和免疫兔子TSIR-1316-LBD + TSIR-4810-LBD产生对抗病毒感染的保护T.豆状通过建立了94.6％，减少96％和80％所证明分别为幼虫。这些数据表明，TsIR-1316-LBD和TsIR-4810-LBD是有前途的候选疫苗或针对猪囊尾rc病的新型药物靶标。