Abstract

The transcriptomic profile associated with osteo- and adipogenic differentiation in growth-arrested multipotent mesenchymal stromal cells (MSCs) from human adipose tissue was analyzed in vitro at 20% (standard laboratory) and 5% (tissue-related) O₂ levels. Compared with day 7, at 5% O₂ on day 14 spontaneous upregulation of osteo- (RUNX2, SP7, BGLAP, and SPP1) and adipogenic differentiation (CEBPA, PPARG, and ADIPOQ) genes in MSCs was observed (p < 0.05). Thus, upon expansion under tissue-related O₂, MSCs demonstrated a bipotent transcriptomic profile, which may contribute to the improvement of their hematopoiesis-supportive function.

中文翻译：

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双能承诺相关基因在不同O2水平下在多能间质基质细胞中的差异表达。

摘要

在体外以20％（标准实验室）和5％（组织相关）O ₂水平分析了与人类脂肪组织相关的生长停滞的多能性间充质基质细胞（MSCs）中与骨形成和脂肪形成分化相关的转录组概况。与第7天相比，第14天在5％O ₂下观察到MSC中的骨（RUNX2，SP7，BGLAP和SPP1）基因和脂肪形成分化（CEBPA，PPARG和ADIPOQ）基因自发上调（p <0.05）。因此，在组织相关的O ₂下膨胀，MSCs显示出双能转录组谱，这可能有助于改善其造血支持功能。