• Plant organellar RNA editing is a distinct type of post‐transcriptional RNA modification that is critical for plant development. We showed previously that the RNA editing factor SlORRM4 is required for mitochondrial function and fruit ripening in tomato (Solanum lycopersicum). However, a comprehensive atlas of the RNA editing mediated by SlORRM4 is lacking.
• We observed that SlORRM4 is targeted to both chloroplasts and mitochondria, and its knockout results in pale‐green leaves and delayed fruit ripening. Using high‐throughput sequencing, we identified 12 chloroplast editing sites and 336 mitochondrial editing sites controlled by SlORRM4, accounting for 23% of chloroplast sites in leaves and 61% of mitochondrial sites in fruits, respectively.
• Analysis of native RNA immunoprecipitation sequencing revealed that SlORRM4 binds to 31 RNA targets; 19 of these targets contain SlORRM4‐dependent editing sites. Large‐scale analysis of putative SlORRM4‐interacting proteins identified SlRIP1b, a RIP/MORF protein. Moreover, functional characterization demonstrated that SlRIP1b is involved in tomato fruit ripening.
• Our results indicate that SlORRM4 binds to RNA targets and interacts with SlRIP1b to broadly affect RNA editing in tomato organelles. These results provide insights into the molecular and functional diversity of RNA editing factors in higher plants.

中文翻译：

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番茄中含有RNA识别基序的蛋白ORRM4介导的细胞器RNA编辑的分子和功能多样性。

• 植物细胞器RNA编辑是转录后RNA修饰的一种独特类型，对植物发育至关重要。我们先前表明，番茄（Solanum lycopersicum）的线粒体功能和果实成熟需要RNA编辑因子SlORRM4 。然而，缺乏由S1ORRM4介导的RNA编辑的全面图谱。
• 我们观察到，SlORRM4既针对叶绿体又针对线粒体，并且其敲除会导致叶片呈浅绿色并延迟果实成熟。使用高通量测序，我们确定了受SlORRM4控制的12个叶绿体编辑位点和336个线粒体编辑位点，分别占叶片中叶绿体位点的23％和水果中线粒体位点的61％。
• 对天然RNA免疫沉淀测序的分析表明，S1ORRM4结合了31个RNA靶标。这些目标中的19个包含与SlORRM4相关的编辑站点。对假定的SlORRM4相互作用蛋白的大规模分析确定了SIPR1b，一种RIP / MORF蛋白。此外，功能表征表明SlRIP1b与番茄果实成熟有关。
• 我们的结果表明，SlORRM4与RNA靶标结合并与SlRIP1b相互作用，从而广泛影响番茄细胞器中的RNA编辑。这些结果提供了对高等植物中RNA编辑因子的分子和功能多样性的见解。