This study aims to make a comparison between different forms of three-dimensional (3D) spheroid U87 tumor cell models that contain fibroblast cells as part of the tumor microenvironment. This purpose is to select the best representative tumor model to be used for further studies to investigate the effects of the anti-invasive pharmacological agents. For this purpose, different options to prepare the 3D spherical invasion model were tested based on the ability of the spheroids to invade in collagen microenvironment. There was a significant positive effect of the fibroblasts on the invasion of the U87 cells in the 3D invasion model compared with the control model that contains U87 cells alone. This result gives proof of the positive effect of the fibroblasts on invasion of tumor cells. Furthermore, these results add more proofs on the importance of adding cells of the tumor microenvironments to the tumor models to make the models more representative to the real in vivo tumor situation.

中文翻译：

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不同3D球形肿瘤侵袭模型之间的比较。

这项研究旨在比较包含成纤维细胞作为肿瘤微环境一部分的三维（3D）球形U87肿瘤细胞模型的不同形式。目的是选择最有代表性的肿瘤模型，以用于进一步的研究，以研究抗侵入药理作用。为此，基于球体侵入胶原蛋白微环境的能力，测试了准备3D球形入侵模型的不同选择。与仅包含U87细胞的对照模型相比，在3D入侵模型中，成纤维细胞对U87细胞的侵袭具有显着的积极作用。该结果证明了成纤维细胞对肿瘤细胞侵袭的积极作用。此外，