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Zirconia-containing wollastonite ceramics derived from biowaste resources for bone tissue engineering.
Biomedical Materials ( IF 3.9 ) Pub Date : 2020-08-06 , DOI: 10.1088/1748-605x/ab975d
Palakurthy Srinath 1 , P Abdul Azeem , K Venugopal Reddy , Vasudevarao Penugurti , Bramanandam Manavathi
Affiliation  

Zirconia-containing wollastonite (CaSiO 3 ) ceramics with partial substitution of zirconia (1, 3 and 5 mol%) were prepared using eggshells and rice husk ash as source materials for calcium oxide and silica, respectively, through a sol–gel technique. The effect of incorporation of zirconia on in vitro bioactivity, mechanical properties, degradability and cytocompatibility of wollastonite was studied. Bioactivity was evaluated by in vitro assay using simulated body fluid while degradability was tested in Tris-HCl buffer solution for different time periods (1, 3, 7, 14 and 21 d) according to the ISO 10 993-14 standard. Human osteosarcoma (MG-63) cells were used to assess cytocompatibility with the MTT assay. X-ray diffractometry, Fourier transform infrared spectroscopy and scanning electron microscopy–energy dispersive spectroscopy were used to characterize the ceramics before and after in vitro studies. The results obtained showed that increasing the ...

中文翻译:

来自生物废物资源的含氧化锆的硅灰石陶瓷用于骨组织工程。

分别使用蛋壳和稻壳灰作为氧化钙和二氧化硅的原料,通过溶胶-凝胶技术制备了部分取代氧化锆(1、3 和 5 mol%)的含氧化锆硅灰石(CaSiO 3 )陶瓷。研究了氧化锆的掺入对硅灰石的体外生物活性、机械性能、降解性和细胞相容性的影响。根据 ISO 10 993-14 标准,使用模拟体液通过体外测定评估生物活性,同时在 Tris-HCl 缓冲溶液中测试不同时间段(1、3、7、14 和 21 天)的降解性。人骨肉瘤 (MG-63) 细胞用于通过 MTT 测定评估细胞相容性。X射线衍射,傅里叶变换红外光谱和扫描电子显微镜 - 能量色散光谱用于在体外研究之前和之后表征陶瓷。结果表明,增加...
更新日期:2020-08-10
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