SIGNIFICANCE Nanosecond-pulsed laser has proven to be used to obtain the velocity of blood using the speckle contrast method. Without the scanning time, it has potential for achieving fast two-dimensional blood flow images in a photoacoustic imaging system with the same pulsed laser. AIM Our study aimed to evaluate the qualities of regional cerebral blood flow (rCBF) obtained in a laser speckle contrast imaging (LSCI) system using continuous wave (cw) and nanosecond pulse laser sources. APPROACH First, a LSCI system consisting of a cw laser with a wavelength of 632.8 nm and a cw laser/nanosecond pulse laser with a wavelength of 532 nm was developed. This system was used to obtain rCBF images of mouse in vivo with two different laser sources. RESULTS Continuous wave lasers (532 and 632.8 nm) show different imaging characteristics for rCBF imaging. The rCBF images obtained using 532-nm nanosecond pulse laser showed higher resolution than those using 532-nm cw laser. There was no significant difference in the results using nanosecond pulse laser among various pulse widths or repetition rates. CONCLUSIONS It is proved that a nanosecond pulse laser could be used for LSCI.

中文翻译：

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使用纳秒脉冲激光源的激光散斑对比度成像系统。


意义 纳秒脉冲激光已被证明可用于通过散斑对比法获得血流速度。如果没有扫描时间，它有可能使用相同的脉冲激光在光声成像系统中实现快速二维血流图像。目的 我们的研究旨在评估使用连续波 (cw) 和纳秒脉冲激光源的激光散斑对比成像 (LSCI) 系统中获得的局部脑血流 (rCBF) 的质量。方法 首先，开发了由波长为 632.8 nm 的连续激光器和波长为 532 nm 的连续激光器/纳秒脉冲激光器组成的 LSCI 系统。该系统用于通过两种不同的激光源获得小鼠体内的 rCBF 图像。结果连续波激光（532 和 632.8 nm）在 rCBF 成像中表现出不同的成像特性。使用 532 nm 纳秒脉冲激光获得的 rCBF 图像比使用 532 nm 连续激光获得的 rCBF 图像显示出更高的分辨率。使用纳秒脉冲激光在不同脉冲宽度或重复频率下的结果没有显着差异。结论 证明纳秒脉冲激光可用于LSCI。