Aberrant expression of LINC00520 has been identified in head and neck squamous carcinoma (HNSCC). However, its function in the radiosensitivity of HNSCC remain unclear. Herein, we aimed to define the role LINC00520 in the radiosensitivity of HNSCC and identify the underlying mechanism. Tumour tissues and adjacent normal tissue were collected from HNSCC patients. Differentially expressed genes (DEGs) in HNSCC tumour were obtained from the cancer genome atlas (TCGA) database. Interactions between LINC00520 and miR-195, homeobox A10 (HOXA10) and miR-195 were evaluated by dual-luciferase reporter gene assay, RNA Immunoprecipitation (RIP), and RNA pull-down assay. The effects of LINC00520/miR-195/HOXA10 on radiosensitivity of HNSCC were analysed in the evaluation of radiotherapy outcome. Cell proliferation, invasion, migration, and apoptosis of HNSCC cells were accessed via gain- and loss-of-function approaches. Tumour xenograft in nude mice was conducted in order to confirm the results in vivo. LINC00520 was upregulated while miR-195 was downregulated in HNSCC cells and tissues. Silencing LINC00520 or overexpressing miR-195 promoted radiosensitivity and inhibited cell proliferation, invasion, migration, and apoptosis in HNSCC. Moreover, these in vitro findings were reproduced in vivo in human HNSCC xenograft in nude mice. LINC00520/miR-195/HOXA10 is involved in the radiosensitivity mediation, providing potential therapeutic target for HNSCC treatment.

LINC00520的异常表达已在头颈部鳞状细胞癌（HNSCC）中得到鉴定。然而，其在HNSCC放射敏感性中的功能仍不清楚。在本文中，我们旨在定义LINC00520在HNSCC的放射敏感性中的作用，并确定其潜在机制。从HNSCC患者中收集肿瘤组织和邻近的正常组织。HNSCC肿瘤中的差异表达基因（DEG）是从癌症基因组图谱（TCGA）数据库中获得的。LINC00520与miR-195，同源框A10（HOXA10）和miR-195之间的相互作用通过双荧光素酶报告基因测定，RNA免疫沉淀（RIP）和RNA下拉测定进行了评估。在评估放射治疗结果时，分析了LINC00520 / miR-195 / HOXA10对HNSCC放射敏感性的影响。细胞增殖，侵袭，迁移，通过功能获得和丧失的方法。为了证实体内结果，进行了裸鼠肿瘤异种移植。在HNSCC细胞和组织中，LINC00520被上调，而miR-195被下调。沉默LINC00520或过度表达miR-195可以促进放射敏感性，并抑制HNSCC中的细胞增殖，侵袭，迁移和凋亡。此外，这些体外研究结果在裸鼠的人类HNSCC异种移植物中体内复制。LINC00520 / miR-195 / HOXA10参与了放射敏感性介导，为HNSCC治疗提供了潜在的治疗靶点。