Introduction. Klebsiella rods, belonging to the family Enterobacteriaceae , are generally opportunistic pathogens commonly associated with nosocomial infections, especially in intensive care units. Interestingly, strains of this genus also show multi-drug resistance. In recent years, multiple studies have indicated that the prevalence of carbapenem resistance has increased rapidly among Klebsiella representatives. Aim. The aim of this study was to assess the usefulness of selected phenotypic and genotypic methods for the detection of the most important carbapenemases in Klebsiella strains. Methodology. The study involved 51 Klebsiella strains. The ability to produce carbapenemases was determined by phenotypic methods (double disc synergy test, test with four discs and three inhibitors, CarbaNP test, culture on chromogenic medium, panels of automatic method – Phoenix, CIM test and modified Hodge test). The potential for carbapenemase synthesis was also evaluated using real-time PCR, detecting bla VIM/IMP, bla KPC, bla NDM and bla OXA-48 genes. Results. Using the phenotypic methods, positive results were obtained for all of the analysed strains. Using PCR, carbapenemase synthesis potential was confirmed on the molecular level; the bla VIM gene was detected in 23 strains, the bla NDM gene in 26 strains and the bla OXA-48 gene in two strains. Conclusion. There was complete agreement between the carbapenemases detected by the genetic method and the results obtained with phenotypic methods.

中文翻译：

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评估所选方法在克雷伯菌中检测碳青霉烯酶的有用性。

介绍。 属于肠杆菌科的克雷伯氏菌杆通常是机会性病原体，通常与医院感染有关，特别是在重症监护病房中。有趣的是，该属的菌株也表现出多重耐药性。近年来，多项研究表明，克雷伯菌属代表中碳青霉烯耐药性的患病率迅速增加。目标。这项研究的目的是评估所选的表型和基因型方法对检测克雷伯菌中最重要的碳青霉烯酶的有用性。方法。该研究涉及51个克雷伯氏菌 株。产生碳青霉烯酶的能力是通过表型方法确定的（双盘协同试验，四盘和三种抑制剂的试验，CarbaNP试验，在生色培养基上的培养，自动方法组– Phoenix，CIM试验和改良的Hodge试验）。还使用实时PCR评估了碳青霉烯酶合成的潜力，检测了bla VIM / IMP，bla KPC，bla NDM和bla OXA-48基因。结果。使用表型方法，所有分析菌株均获得阳性结果。使用PCR，在分子水平上证实了碳青霉烯酶的合成潜力；在BLA VIM在23个菌株中检测到该基因，在26个菌株中检测到bla NDM基因，在两个菌株中检测到bla OXA-48基因。结论。遗传方法检测到的碳青霉烯酶与表型方法获得的结果完全一致。