Background. Immune-related pancytopenia (IRP) is a kind of autoimmune disease mediated by autoantibodies in bone marrow. T helper 9 (Th9) cell is a new subset of T cell discovered recently, which mainly expresses cytokine interleukin-9 (IL-9) to exert immune function. Th9 cells are associated with a variety of inflammatory diseases, but the role of Th9 cells in IRP remains unclear. Methods. Fifty patients with IRP and 20 healthy controls were enrolled. The percentage of Th9 cells was detected by flow cytometry (FCM) and ELISA. CD4⁺ lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. Results. The percentage of Th9 cells in CD3⁺CD4⁺ cells was % in the untreated group, which was significantly higher than those in the remission group () () and the control group () (). And that in the remission group was significantly higher than that in the control group (). The level of IL-9 in the untreated group was  pg/mL, which was significantly higher than that in the remission group ( pg/mL) () and control group ( pg/mL) (). That in the remission group was also significantly higher than that in the control group (). They were negatively correlated with hemoglobin, red blood cell, white blood cell, and platelet counts and positively correlated with the percentage of CD19⁺B cells and CD5⁺CD19⁺/CD19⁺B cells, respectively. The mRNA expression levels of PU.1 and BATF in IRP patients were higher than those in controls (). Conclusions. The percentage of Th9 cells in the peripheral blood and the level of IL-9 in the serum of patients with IRP were increased, which was related to the severity of the disease.

中文翻译：

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免疫相关全血细胞减少症患者外周血中的 Th9 细胞增加。

背景。免疫相关性全血细胞减少症（IRP）是一种由骨髓中自身抗体介导的自身免疫性疾病。T辅助9（Th9）细胞是最近发现的一个新的T细胞亚群，主要表达细胞因子白细胞介素9（IL-9）发挥免疫功能。Th9 细胞与多种炎症性疾病有关，但 Th9 细胞在 IRP 中的作用仍不清楚。方法。招募了 50 名 IRP 患者和 20 名健康对照。通过流式细胞术（FCM）和ELISA检测Th9细胞的百分比。磁珠分选CD4 ⁺淋巴细胞，RT-PCR检测Th9细胞相关转录因子PU.1和BATF的mRNA表达水平。结果。^{CD3 +}中Th9细胞的百分比CD4 ⁺细胞是%，未治疗组显着高于缓解组（) ()和对照组 () (）。且缓解组明显高于对照组（）。未治疗组IL-9水平为 pg/mL，显着高于缓解组（ 皮克/毫升) ()和对照组 ( 皮克/毫升) (）。缓解组也显着高于对照组（）。它们与血红蛋白、红细胞、白细胞和血小板计数呈负相关，分别与CD19 ⁺ B细胞和CD5 ⁺ CD19 ⁺ /CD19 ⁺ B细胞的百分比呈正相关。IRP患者PU.1和BATF mRNA表达水平高于对照组（）。 结论。IRP患者外周血Th9细胞百分比和血清IL-9水平升高，与病情严重程度有关。