This study identified 57 basic leucine zipper (bZIP) genes from the pineapple genome, and the analysis of these bZIP genes was focused on the evolution and divergence after multiple duplication events in relation to the pineapple genome fusion. According to bioinformatics analysis of a phylogenetic tree, the bZIP gene family was divided into 11 subgroups in pineapple, Arabidopsis, and rice; gene structure and conserved motif analyses showed that bZIP genes within the same subgroup shared similar intron-exon organizations and motif composition. Further synteny analysis showed 17 segmental duplication events with 27 bZIP genes. The study also analyzed the pineapple gene expression of bZIP genes in different tissues, organs, and developmental stages, as well as in abiotic stress responses. The RNA-sequencing data showed that AcobZIP57 was upregulated in all tissues, including vegetative and reproductive tissues. AcobZIP28 and AcobZIP43 together with the other 25 bZIP genes did not show high expression levels in any tissue. Six bZIP genes were exposed to abiotic stress, and the relative expression levels were detected by quantitative real-time PCR. A significant response was observed for AcobZIP24 against all kinds of abiotic stresses at 24 and 48 h in pineapple root tissues. Our study provides a perspective for the evolutionary history and general biological involvement of the bZIP gene family of pineapple, which laid the foundation for future functional characterization of the bZIP genes in pineapple.

中文翻译：

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菠萝中基本亮氨酸拉链转录因子家族的全基因组分析，表征和表达谱。

这项研究从菠萝基因组中鉴定出57个基本的亮氨酸拉链（bZIP）基因，对这些bZIP基因的分析着重于与菠萝基因组融合相关的多次重复事件后的进化和分化。根据系统发育树的生物信息学分析，bZIP基因家族被分为菠萝，拟南芥中的11个亚组。和米饭；基因结构和保守基序分析表明，同一亚组内的bZIP基因共享相似的内含子-外显子组织和基序组成。进一步的语气分析显示了带有27个bZIP基因的17个片段重复事件。该研究还分析了bZIP基因在不同组织，器官和发育阶段以及非生物应激反应中的菠萝基因表达。RNA测序数据显示AcobZIP57在包括营养和生殖组织在内的所有组织中均上调。AcobZIP28和AcobZIP43连同其他25个bZIP基因在任何组织中均未显示高表达水平。六个bZIP基因暴露于非生物胁迫下，并通过定量实时PCR检测相对表达水平。在菠萝根组织中，在24和48 h观察到AcobZIP24对各种非生物胁迫均具有显着响应。我们的研究为菠萝bZIP基因家族的进化历史和一般生物学参与提供了一个视角，为菠萝​​bZIP基因的未来功能表征奠定了基础。