Purpose: Cancer is one of the most common causes of mortality and morbidity worldwide. Vaccines have been emerged as an attractive approach for their capacity of eliciting long-term immune response targeting cancer cells. Attenuated avirulent Toxoplasma gondii stimulate immunity and activate antitumor cells thereby eliciting rejection of some established cancer. The purpose of this study was to evaluate the antitumor-protective capacity of vaccination with gamma radiation-attenuated T. gondii against ovarian penetration in Ehrlich ascites carcinoma (EAC)-bearing mice.

Materials and methods: Forty-five mice were randomly divided into three groups as follows: nontumor-bearing (normal control); EAC-bearing group (EAC); and mice vaccinated orally with gamma radiation-attenuated T. gondii then inoculated 2 weeks later with EAC (TG + EAC). Survival rate, serum interleukin-12 (IL-12), and levels of IFN-γ mRNA, CD4, and CD8 in ovarian tissues homogenate were assessed. Also, ovarian histopathology and immunohistochemical expressions of metalloproteinase-2, CD34, and vimentin were determined.

Results: The group vaccinated with attenuated T. gondii showed significantly increased survival rates, serum IL-12, and levels of IFN-γ, CD4, and CD8 in ovarian tissue homogenates as well as an enhancement of histopathological and immunohistochemical changes compared to EAC-bearing group.

Conclusion: Vaccination with gamma radiation-attenuated T. gondii has the capacity to supply immunoprotective impact against ovarian invasion in EAC-bearing mice.

目的：癌症是全世界最常见的死亡率和发病率原因之一。疫苗已成为引起针对癌细胞的长期免疫应答的能力的有吸引力的方法。弱毒的弓形虫刺激免疫力并激活抗肿瘤细胞，从而引起某些已建立的癌症的排斥。这项研究的目的是评估在携带Ehrlich腹水癌（EAC）的小鼠中，γ射线减毒的弓形虫疫苗接种对卵巢渗透的抗肿瘤保护能力。

材料和方法： 45只小鼠随机分为三组，即非荷瘤小鼠（正常对照组）和非荷瘤小鼠（正常对照组）。EAC轴承组（EAC）；小鼠经伽马射线减毒弓形虫口服疫苗接种，两周后用EAC（TG + EAC）接种。评估卵巢组织匀浆的存活率，血清白介素12（IL-12）以及IFN-γmRNA，CD4和CD8的水平。此外，确定了卵巢组织病理学和金属蛋白酶2，CD34和波形蛋白的免疫组织化学表达。

结果：接种减毒弓形虫的组与EAC-组相比，卵巢组织匀浆中的存活率，血清IL-12以及IFN-γ，CD4和CD8的水平显着提高，并且组织病理学和免疫组织化学变化增强。轴承组。

结论：用γ射线减毒的弓形虫进行的疫苗接种能够提供免疫保护作用，抵抗荷EAC小鼠的卵巢侵袭。