This study aimed at designing a novel electrospun scaffolding material that structurally and chemically resembles native extracellular matrix for bone tissue engineering. Calcium chloride-complexed polyamide66 (PA66/CaCl₂) and pure PA66 electrospun nanofibers were fabricated by the electrospinning method. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared (FTIR) spectroscopy were used to investigate the effect of the presence of ionized salt in the polymer solution on the mechanical properties and other properties of the electrospun scaffolds. The results show that addition of CaCl₂ to PA66 solution can achieve an internal modification and improve the tensile strength and modulus of the polymeric electrospun nanofiber. Ca²⁺ uploaded through electrospun fibers could provide nucleation sites for the formation of hydroxylapatite (HA) coating. After mineralization, the tensile strength and modulus of HA/PA66/CaCl₂ scaffolds reach up to 41.33 ± 16.17 MPa and 168.59 ± 42.20 MPa, respectively. LIVE/DEAD assay shows that compared with pure PA66 scaffolds, a greater density of viable MC3T3-E1 cells were seen on the HA/PA66/CaCl₂ scaffolds. Cell Counting Kit-8 results indicate that HA/PA66/CaCl₂ scaffolds displays a more favorable ability to promote MC3T3-E1 cell proliferation and growth than that of the other groups with the prolongation of culture time. These results demonstrate that HA/PA66/CaCl₂ scaffolds that structurally and chemically resemble native bone have a good cytocompatibility, and might be a potential candidate for bone tissue engineering.

中文翻译：

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用于骨组织工程的矿化聚酰胺66 /氯化钙纳米纤维。

这项研究旨在设计一种新型的电纺支架材料，该材料在结构和化学上类似于天然的细胞外基质，用于骨组织工程。通过电纺丝法制备了氯化钙复合聚酰胺66（PA66 / CaCl ₂）和纯PA66电纺纳米纤维。扫描电子显微镜，X射线衍射和傅立叶变换红外（FTIR）光谱用于研究聚合物溶液中电离盐的存在对电纺支架的机械性能和其他性能的影响。结果表明，向PA66溶液中添加CaCl ₂可以实现内部改性，并提高聚合物电纺纳米纤维的拉伸强度和模量。钙²⁺通过静电纺丝纤维上载可为形成羟基磷灰石（HA）涂层提供成核位置。矿化后，HA / PA66 / CaCl ₂支架的拉伸强度和模量分别达到41.33±16.17 MPa和168.59±42.20 MPa。LIVE / DEAD分析显示，与纯PA66支架相比，在HA / PA66 / CaCl ₂支架上观察到更大密度的活MC3T3-E1细胞。Cell Counting Kit-8结果表明，随着培养时间的延长，与其他组相比，HA / PA66 / CaCl ₂支架具有更好的促进MC3T3-E1细胞增殖和生长的能力。这些结果表明，HA / PA66 / CaCl ₂ 结构和化学上类似于天然骨的支架具有良好的细胞相容性，并且可能是骨组织工程的潜在候选者。