Honey bee larva powder (HLP) has traditionally been used as a daily supplement and tonic for health promotion with an uncertain scientific basis. In this study, B16-F10 tumor-bearing mice were established to evaluate the immunomodulatory activity of HLP. The proliferation and apoptosis assays were performed to evaluate the anti-inflammatory activity of honey bee larva extract (HLE) in RAW 264.7 macrophage. The in vivo experimental results demonstrated that the oral administration of freeze-dried HLP (4 and 6 g/kg) significantly enhanced the spleen index, the percentage of CD4⁺cells, and the ratio of CD4⁺ and CD8⁺ T lymphocytes (CD4⁺/CD8⁺) in the peripheral blood compared with those in the tumor control mice. The in vitro studies demonstrated the potent immunomodulatory activities of HLE through the induction of RAW 264.7 macrophage proliferation and the mitigation of doxorubicin (DOX)-induced toxicity. HLE also exhibits anti-inflammatory activity by decreasing the production of nitric oxide (NO) and the cytokine level of interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage. The present study provides important scientific evidence for the immunomodulatory and anti-inflammatory activities of HLP and HLE.

中文翻译：

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蜜蜂幼虫粉的免疫调节和消炎活性评估。

传统上，蜜蜂幼虫粉（HLP）被用作日常补充剂和滋补品以促进健康，但其科学依据尚不确定。在这项研究中，建立了B16-F10荷瘤小鼠以评估HLP的免疫调节活性。进行增殖和凋亡测定以评估蜜蜂幼虫提取物（HLE）在RAW 264.7巨噬细胞中的抗炎活性。的体内实验结果表明，冷冻干燥的HLP的口服给药（4和6克/千克）显著增强脾脏指数，CD4的百分比⁺细胞和CD4的比例⁺和CD8 ⁺ T淋巴细胞（CD4 ⁺ / CD8 ⁺）与肿瘤对照小鼠的外周血相比）。该体外研究通过RAW 264.7巨噬细胞增殖的诱导和多柔比星（DOX）诱导的毒性的减轻证实HLE的强效免疫调节活性。HLE还通过降低一氧化氮（NO）的产生和脂多糖（LPS）刺激的RAW 264.7巨噬细胞中白细胞介素6（IL-6）的细胞因子水平而表现出抗炎活性。本研究为HLP和HLE的免疫调节和抗炎活性提供了重要的科学证据。