ADP-ribosylation factor (Arf) GTPase-activating protein (GAP) with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) is an Arf6 GAP that regulates membrane trafficking and is critical for the migratory potential of cells. However, the roles of ACAP1 have not been fully explored and its association with clinicopathological features in ovarian cancer is still unknown. In this study, we systematically analyzed multiple databases, including TISIDB, Tumor Immune Estimation Resource (TIMER2.0), Gene Expression Omnibus (GEO), CORTECON, Kaplan–Meier Plotter and LinkedOmics platforms to reveal the clinical significance and function of ACAP1 in ovarian cancer. We found that the expression of ACAP1 was upregulated in ovarian cancer and high ACAP1 expression predicted poor prognosis. Our data demonstrated that the expression and methylation status of ACAP1 were strongly correlated with immune infiltration levels, immunomodulators, and chemokines. Gene set enrichment analysis (GSEA) analysis also showed that the mechanism of ACAP1 in regulating ovarian cancer was related to a variety of immune-related pathways. In addition, we also revealed that the expression of ACAP1 was altered during cell differentiation and associated with cancer cell stemness markers. Our study highlights the clinical significance of ACAP1 in ovarian cancer and provides insight into the novel function of ACAP1 in regulation of tumor immune microenvironment and cancer cell stemness.

中文翻译：

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ACAP1的表达与卵巢癌的肿瘤免疫浸润和临床结果有关。

具有卷曲螺旋，锚蛋白重复序列​​和PH结构域1（ACAP1）的ADP-核糖基化因子（Arf）GTP酶激活蛋白（GAP）是Arf6 GAP，它调节膜运输，对细胞的迁移潜力至关重要。然而，ACAP1的作用尚未得到充分探讨，其与卵巢癌临床病理特征的关联仍是未知的。在这项研究中，我们系统地分析了多个数据库，包括TISIDB，肿瘤免疫估计资源（TIMER2.0），基因表达综合（GEO），CORTECON，Kaplan-Meier绘图仪和LinkedOmics平台，以揭示ACAP1在卵巢癌中的临床意义和功能。癌症。我们发现卵巢癌中ACAP1的表达上调，而高ACAP1的表达预后不良。我们的数据表明，ACAP1的表达和甲基化状态与免疫浸润水平，免疫调节剂和趋化因子密切相关。基因集富集分析（GSEA）分析还显示，ACAP1调控卵巢癌的机制与多种免疫相关途径有关。此外，我们还揭示了ACAP1的表达在细胞分化过程中发生了改变，并与癌细胞干性标志物相关。我们的研究突出了ACAP1在卵巢癌中的临床意义，并为ACAP1在调节肿瘤免疫微环境和癌细胞干性方面的新型功能提供了见识。基因集富集分析（GSEA）分析还显示，ACAP1调控卵巢癌的机制与多种免疫相关途径有关。此外，我们还揭示了ACAP1的表达在细胞分化过程中发生了改变，并与癌细胞干性标志物相关。我们的研究突出了ACAP1在卵巢癌中的临床意义，并为ACAP1在调节肿瘤免疫微环境和癌细胞干性方面的新型功能提供了见识。基因集富集分析（GSEA）分析还显示，ACAP1调控卵巢癌的机制与多种免疫相关途径有关。此外，我们还揭示了ACAP1的表达在细胞分化过程中发生了改变，并与癌细胞干性标志物相关。我们的研究突出了ACAP1在卵巢癌中的临床意义，并为ACAP1在调节肿瘤免疫微环境和癌细胞干性方面的新型功能提供了见识。