The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maximum inhibitory effect equivalent to 24.34% and 26.81% was recorded when Apt17 was co-incubated with S. enteritidis TM 6 and S. enteritidis TM 68 respectively for 13 h. The inhibitory effect of Apt17 was also confirmed with Triphenyl Tetrazolium Chloride. Under Scanning Electron Microscope, the presence of Apt17 resulted in altered three dimensional structure. While the treated cells of S. enteritidis TM 6 were arranged as monolayers, the sessile aggregates of S. enteritidis TM 68 appeared thinner and exhibited less surface coverage when compared to control. Moreover, the treated cells lost their exopolysaccharide matrix. The co-incubation of Apt17 with ampicillin MIC/10 for 24 h, inhibited the biofilms of S. enteritidis TM 6 and S. enteritidis TM 68 by 12.5 and 20.9% respectively. This study demonstrated quantitative and qualitative antibiofilm effect of Apt17 against the biofilms of two Salmonella enteritidis strains. According to our knowledge, this is the first study employing an aptamer that targets SipA protein to inhibit biofilm formation in Salmonella.

中文翻译：

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沙门氏菌侵袭蛋白靶向适体抑制肠炎沙门氏菌生物膜

目前的研究旨在评估针对沙门氏菌入侵蛋白 A (SipA) 的 DNA 适体 (Apt17) 的抑制作用。Apt17 对两种肠炎沙门氏菌菌株的生物膜形成的影响，在不同亚 MIC 浓度下单独或与氨苄青霉素组合进行测试。当 Apt17 分别与肠炎沙门氏菌 TM 6 和肠炎沙门氏菌 TM 68 共同孵育 13 小时时，记录的最大抑制作用相当于 24.34% 和 26.81%。Apt17 的抑制作用也用 Triphenyl Tetrazolium Chloride 证实。在扫描电子显微镜下，Apt17 的存在导致了三维结构的改变。虽然经处理的肠炎沙门氏菌 TM 6 细胞排列为单层，但 S. enteritidis TM 6 的固着聚集体。与对照相比，enteritidis TM 68 显得更薄，表面覆盖率更低。此外，处理过的细胞失去了它们的胞外多糖基质。Apt17 与氨苄青霉素 MIC/10 共孵育 24 小时，分别抑制了肠炎沙门氏菌 TM 6 和肠炎沙门氏菌 TM 68 的生物膜 12.5% 和 20.9%。该研究证明了 Apt17 对两种肠炎沙门氏菌菌株的生物膜的定量和定性抗生物膜作用。据我们所知，这是第一项使用适体靶向 SipA 蛋白来抑制沙门氏菌生物膜形成的研究。该研究证明了 Apt17 对两种肠炎沙门氏菌菌株的生物膜的定量和定性抗生物膜作用。据我们所知，这是第一项使用适体靶向 SipA 蛋白来抑制沙门氏菌生物膜形成的研究。该研究证明了 Apt17 对两种肠炎沙门氏菌菌株的生物膜的定量和定性抗生物膜作用。据我们所知，这是第一项使用适体靶向 SipA 蛋白来抑制沙门氏菌生物膜形成的研究。