Abstract

The current study identified the specific antibodies that recognise amyloid protein for Alzheimer disease – immunotherapy. The immune-selection of random sequences from a phage display library and sequencing to obtain the random 12 amino acids peptide library for each antibody, and then we analysed these peptides for unique and common sequences, relation to Aβ42 sequence and shape and pattern of the amino acid reaction to the antibody to predict the epitopes. Data obtained for 4G8 showed that, the sequence segment related to the putative epitope of 4G8 was LVFFAED. Nine of the ten top sequences contain the sequence RHD corresponding to the Aβ sequence from residues 5-7. Peptide 7 has the sequence IRYDTGSYHIH, which has a RYD. It was concluded that, 4G8 and 6E10 can tolerate the binding the sequences that explain it is able to recognise amyloid aggregates.

摘要

目前的研究确定了识别阿尔茨海默病淀粉样蛋白的特异性抗体——免疫疗法。从噬菌体展示文库中免疫选择随机序列并测序以获得每个抗体的随机 12 个氨基酸肽库，然后我们分析这些肽的独特和共同序列、与 Aβ42 序列的关系以及氨基的形状和模式对抗体的酸反应以预测表位。获得的4G8数据表明，与4G8的推定表位相关的序列片段是LVFFAED。十个顶部序列中的九个包含对应于残基 5-7 的 Aβ 序列的序列 RHD。肽 7 具有序列 IRYDTGSYHIH，其具有 RYD。得出的结论是，