MiR-125 has been shown to be involved in a variety of cancers, including cervical cancer (CC). Here, our goal was to explore miR-125 functional role and molecular mechanism in cervical cancer development and progression. qRT-PCR was employ to detect miR-125 and VEGF mRNA expression. Western blot was applied for testing protein levels (VEGF, E-cadherin, N-cadherin, vimentin, AKT, p-AKT, PI3K, and p-PI3K). MTT and transwell assays were used for detecting cervical cancer cell progression, including cell viability, migration, and invasion. We observed that miR-125 was downregulated, whereas VEGF was upregulated in cervical cancer tissues and cell lines (CaSki and SiHa). MiR-125 inhibited the proliferation, invasion, and migration by targeting VEGF in cervical cancer. Moreover, miR-125 negatively regulated VEGF expression in cervical cancer tissues. Finally, we demonstrated that miR-520d-5p inhibited the activation of PI3K/AKT signaling pathway. In conclusion, the findings demonstrated that miR-125 inhibited cervical cancer progression and development by suppression VEGF and PI3K/AKT signaling pathway.

中文翻译：

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MiR-125通过调节VEGF和PI3K / AKT信号通路抑制宫颈癌的进展。

已证明MiR-125与多种癌症有关，包括宫颈癌（CC）。在这里，我们的目标是探索miR-125在宫颈癌发展和进程中的功能作用和分子机制。采用qRT-PCR检测miR-125和VEGF mRNA的表达。Western blot用于检测蛋白质水平（VEGF，E-钙粘蛋白，N-钙粘蛋白，波形蛋白，AKT，p-AKT，PI3K和p-PI3K）。MTT和Transwell分析用于检测子宫颈癌细胞的进程，包括细胞活力，迁移和侵袭。我们观察到，miR-125在宫颈癌组织和细胞系（CaSki和SiHa）中被下调，而VEGF被上调。MiR-125通过靶向VEGF在宫颈癌中抑制增殖，侵袭和迁移。而且，miR-125负调节宫颈癌组织中的VEGF表达。最后，我们证明了miR-520d-5p抑制了PI3K / AKT信号通路的激活。总之，研究结果表明miR-125通过抑制VEGF和PI3K / AKT信号通路抑制宫颈癌的进展和发展。