In plants, the vegetative to reproductive growth phase transition (termed bolting in Arabidopsis) generally precedes age-dependent leaf senescence (LS). Many studies describe a temporal link between bolting time and LS, as plants that bolt early, senesce early, and plants that bolt late, senesce late. However, the molecular mechanisms underlying this relationship are unknown and are potentially agriculturally important, as they may allow for the development of crops that can overcome early LS caused by stress-related early bolting. We hypothesized that gene expression changes associated with bolting time were regulating LS. We used a mutant that displays both early bolting and early LS as a model to test this hypothesis. An RNA-seq time series experiment was completed to compare the early bolting mutant to vegetative WT plants of the same age. This allowed us to isolate bolting time-associated genes (BAGs) expressed in an older rosette leaf at the time of inflorescence emergence. The BAG list contains many well characterized LS regulators (ORE1, WRKY45, WRKY28), and GO analysis revealed enrichment for LS and LS-related processes. These bolting associated LS regulators likely contribute to the temporal coupling of bolting time to LS.

中文翻译：

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拟南芥叶片抽escence时基因表达的变化与叶片衰老相关

在植物中，从植物生长到生殖生长的过渡（在拟南芥中称为“抽ing”）通常先于年龄相关的叶片衰老（LS）。许多研究都描述了抽time时间和LS之间的时间联系，即早插早衰的植株和抽插晚衰的植株。但是，这种关系的分子机制尚不清楚，并且在农业上可能具有重要意义，因为它们可以促进作物生长，从而克服因与压力有关的早期抽early而导致的早期LS。我们假设与抽ing时间相关的基因表达变化正在调节LS。我们使用了既显示早期螺栓连接又显示早期LS的突变体作为模型来检验该假设。完成了一个RNA-seq时间序列实验，将早期的螺栓突变体与相同年龄的无性野生植物进行了比较。这使我们能够分离出在花序出苗时在较老的莲座丛叶中表达的bolt插时间相关基因（BAG）。BAG列表包含许多特性良好的LS调节器（ORE1，WRKY45，WRKY28），GO分析显示，LS和与LS相关的过程富集了。这些与螺栓连接的LS调节器可能有助于将螺栓连接时间临时连接到LS。