The hippocampus and associated cholinergic inputs regulate spatial memory in rodents. Muscarinic blockade with scopolamine results in cognition deficits usually attributed to impaired memory encoding, but effects on memory retrieval are controversial. Here, we simultaneously recorded hundreds of neurons in mouse hippocampal CA1 using calcium imaging with a miniatured fluorescent microscope to study place cell and ensemble neuronal properties in a linear track environment. We found decoding accuracy and ensemble stability were significantly reduced after the administration of scopolamine. Several other parameters including the 〖Ca〗^(2+) event rate, number of total cells and place cells observed, spatial information content were affected including a small increase in running speed. This study enhances the understanding of cholinergic blockade on spatial memory impairment.

中文翻译：

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用微型显微镜观察东Moving碱对自由移动小鼠海马CA1细胞和网络特性的影响

海马和相关的胆碱能输入调节啮齿动物的空间记忆。用东pol碱进行毒蕈碱性阻断可导致认知障碍，通常归因于记忆编码受损，但对记忆检索的影响尚存争议。在这里，我们同时使用微型荧光显微镜通过钙成像技术在小鼠海马CA1中记录了数百个神经元，以研究线性轨道环境中的细胞和集合神经元特性。我们发现东碱给药后，解码准确性和合奏稳定性显着降低。包括〖Ca〗^（2+）事件发生率，观察到的总细胞数和位置细胞数，空间信息内容在内的其他几个参数也受到影响，包括运行速度的小幅提高。