Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. We developed a molecular diagnostic test for SARS-CoV-2, FIND (Fast Isothermal Nucleic acid Detection), based on an enhanced isothermal recombinase polymerase amplification reaction. FIND has a detection limit on patient samples close to that of RT-qPCR, requires minimal instrumentation, and is highly scalable and cheap. It can be performed in high throughput, does not cross-react with other common coronaviruses, avoids bottlenecks caused by the current worldwide shortage of RNA isolation kits, and takes ~45 minutes from sample collection to results. FIND can be adapted to future novel viruses in days once sequence is available.

中文翻译：

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用于病毒检测的增强等温扩增测定。

快速、廉价、可靠的诊断对于控制传染病的传播至关重要。当前最先进的诊断技术具有高度敏感性和特异性，但速度较慢，并且需要昂贵的设备。我们基于增强的等温重组酶聚合酶扩增反应开发了 SARS-CoV-2 的分子诊断测试 FIND（快速等温核酸检测）。FIND 对患者样本的检测限接近 RT-qPCR，需要最少的仪器，并且具有高度可扩展性和廉价性。它可以高通量进行，不与其他常见的冠状病毒发生交叉反应，避免了当前全球范围内 RNA 分离试剂盒短缺造成的瓶颈，从样本收集到结果大约需要 45 分钟。一旦序列可用，FIND 可以在几天内适应未来的新型病毒。