Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is associated with a wide range of systemic manifestations. Several observations support a role for vascular endothelial dysfunction in the pathogenesis including an increased incidence of thrombotic events and coagulopathy and the presence of vascular risk factors as an independent predictor of poor prognosis. It has recently been reported that endothelitis is associated with viral inclusion bodies suggesting a direct role for SARS-CoV-2 in the pathogenesis. The ACE2 receptor has been shown to mediate SARS-CoV-2 uptake and it has been proposed that CD147 (BSG) can function as an alternative cell surface receptor. To define the endothelial cell populations that are susceptible to infection with SARS-CoV-2, we investigated the expression of ACE2 as well as other genes implicated in the cellular entry of SARS-Cov-2 in the vascular endothelium through the analysis of single cell sequencing data derived from multiple human tissues (skin, liver, kidney, lung and intestine). We found that CD147 (BSG) but not ACE2 is detectable in vascular endothelial cells within single cell sequencing datasets derived from multiple tissues in healthy individuals. This implies that either ACE2 is not expressed in healthy tissue but is instead induced in response to SARS-Cov2 or that SARS-Cov2 enters endothelial cells via an alternative receptor such as CD147.

中文翻译：

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在健康个体的多个组织衍生的单细胞RNA测序数据集中，在血管内皮细胞中可检测到CD147（BSG）但未检测到ACE2表达

冠状病毒病2019（COVID-19）由严重的急性呼吸系统综合症冠状病毒2（SARS-CoV-2）引起，并与广泛的全身表现有关。一些观察结果支持血管内皮功能障碍在发病机制中的作用，包括血栓形成事件和凝血病的发生率增加，以及血管危险因素的存在是预后不良的独立预测因素。最近有报道说，内皮炎与病毒包涵体有关，提示SARS-CoV-2在发病机理中具有直接作用。已经显示ACE2受体介导SARS-CoV-2摄取，并且已经提出CD147（BSG）可以用作替代的细胞表面受体。为了定义易于感染SARS-CoV-2的内皮细胞群，我们通过分析来自多个人体组织（皮肤，肝，肾，肺和肠）的单细胞测序数据，研究了ACE2以及与SARS-Cov-2进入血管内皮细胞的细胞有关的其他基因的表达。我们发现CD147（BSG）但不是ACE2在健康个体的多个组织衍生的单细胞测序数据集中的血管内皮细胞中可检测到。这意味着ACE2在健康组织中不表达，而是响应SARS-Cov2而被诱导，或者SARS-Cov2通过其他受体（例如CD147）进入内皮细胞。肺和肠）。我们发现CD147（BSG）但不是ACE2在健康个体的多个组织衍生的单细胞测序数据集中的血管内皮细胞中可检测到。这意味着ACE2在健康组织中不表达，而是响应SARS-Cov2而被诱导，或者SARS-Cov2通过其他受体（例如CD147）进入内皮细胞。肺和肠）。我们发现CD147（BSG）但不是ACE2在健康个体的多个组织衍生的单细胞测序数据集中的血管内皮细胞中可检测到。这意味着ACE2在健康组织中不表达，而是响应SARS-Cov2而被诱导，或者SARS-Cov2通过其他受体（例如CD147）进入内皮细胞。